冷冻大熊猫初乳XO和PAO活性测定及其与牛、羊乳的比较  

Determination of XO and PAO Activities of Frozen Giant Panda Colostrum and Its Comparison with Cow and Goat Milk

作　　者：付尚辰 雷颖虎 赵鹏鹏 张丹辉 沈洁娜 刘永峰[1] FU Shangchen;LEI Yinghu;ZHAO Pengpeng;ZHANG Danhui;SHEN Jiena;LIU Yongfeng(College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710100,China;Research Center for Qinling Giant Panda(Shaanxi Rare Wildlife Rescue Base),Xi'an 710032,China)

机构地区：[1]陕西师范大学食品工程与营养科学学院,西安710100 [2]秦岭大熊猫研究中心(陕西省珍稀野生动物救护基地),西安710032

出　　处：《西北农业学报》2022年第10期1258-1265,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省林业科学院创新团队建设项目(SXLK2020-0303);陕西省林业科技创新计划专项(SXLK2021-0221);陕西省重点研发计划(2019ZDLNY06-05);中央高校基本科研业务费(GK202001002)。

摘　　要：黄嘌呤氧化酶(XO)和多胺氧化酶(PAO)是乳汁中两类重要的氧化还原酶,与新生幼崽免疫系统的形成密切相关。采用牛、羊鲜乳及其冷冻乳建立XO和PAO活性的AmplexRed荧光检测方法,对冷冻大熊猫初乳中XO和PAO活性进行测定,并与新鲜和冷冻牛、羊乳中这两种酶的活性进行比较。结果表明,3种乳中XO活性高于PAO活性;XO活性总体趋势为牛乳>大熊猫初乳>羊乳;PAO活性总体趋势为牛乳>羊乳>大熊猫初乳。大熊猫初乳通过XO、PAO和乳过氧化物酶LPO系统的协同抗菌机制在新生幼崽肠道微生物和先天免疫系统形成过程中发挥作用且以XO为主。Xanthine oxidase(XO)and Polyamine oxidase(PAO)are two important oxidoreductases in milk,which are closely related to the formation of the immune system in newborns.Amplex Red fluo-rescence assay for establishing XO and PAO activities by using fresh and frozen cow and goat milk was used to determine the activities of XO and PAO in frozen giant panda colostrum,and it was also compared with XO and PAO in fresh and frozen cow and goat milk.The results showed that the XO activities were higher than PAO activities in the three kinds of milk.The XO activities of the three kinds of milk were cow milk,giant panda colostrum and goat milk from order of high to low.The overall trend of PAO activities from high to low were cow milk,goat milk and giant panda colostrum.The synergistic antibacterial mechanism of XO,PAO and LPO systems in giant panda colostrum is dominated by XO,which plays a role in the formation of intestinal microorganism and innate im mune:system in newborn cubs.

关 键 词：大熊猫 冷冻乳 黄嘌呤氧化酶(XO) 多胺氧化酶(PAO) 

分 类 号：S865.3[农业科学—野生动物驯养] Q544.9[农业科学—畜牧兽医]