餐厨垃圾厌氧发酵产沼气过程中的微生物群落结构解析  

ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE IN BIOGAS PRODUCTION BY ANAEROBIC FERMENTATION OF KITCHEN WASTE

作　　者：纪栋 姚志松 张陈 魏志豪 宗正玲 苏有勇[1] Ji Dong;Yao Zhisong;Zhang Chen;Wei Zhihao;Zong Zhengling;Su Youyong(Faculty of Agricultural and Food,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学农业与食品学院,昆明650500

出　　处：《太阳能学报》2022年第9期354-362,共9页Acta Energiae Solaris Sinica

基　　金：国家自然科学基金(51466004)。

摘　　要：以餐厨垃圾为原料,分别进行批量和半连续厌氧发酵,并对微生物进行Miseq高通量测序,结果表明:在餐厨垃圾批量厌氧发酵情况下,最佳发酵温度为35~40℃,最佳发酵浓度约为8%。在餐厨垃圾半连续厌氧发酵情况下,最佳发酵温度约为35℃,发酵系统的最佳有机负荷在2.50~3.00 g VS/(L·d)之间。通过高通量测序分析,发现有Synergistaceae、Porphyromonadaceae、Rikenellaceae、Clostridiaceae_1等细菌种群为厌氧发酵系统相对丰度较高的菌群,有Methanosarcinaceae、Methanomicrobiaceae、Methanosaetaceae等古菌种群为厌氧发酵系统相对丰度较高的菌群,但在批量和半连续发酵体系中的含量均有所差别,且不同温度下的优势微生物种群也不尽相同。In this study,kitchen waste was used as raw material to carry out batch fermentation and semi-continuous fermentation,and high-throughput sequencing of microbial species diversity was conducted,and then the research conclusions were drawn. In the batch fermentation of kitchen waste,the optimal fermentation temperature is between 35 ℃ and 40 ℃,and the optimal fermentation concentration is about 8%. In the semi-continuous fermentation of kitchen waste,the optimal fermentation temperature is about 35 ℃,and the optimal organic load of the fermentation system is between 2.50 g VS/(L·d)and 3.00 g VS/(L·d). Through high-throughput sequencing analysis,it was found that Synergistaceae,Porphyromonadaceae,Rikenellaceae,Clostridiaceae_1 and other bacterial populations had relatively high abundance in the anaerobic fermentation system,while Methanosarcinaceae,Methanomicrobiaceae,Methanosaetaceae and other ancient bacterial populations had relatively high abundance in the anaerobic fermentation system. However,the contents in batch and semi-continuous fermentation systems were different,and the dominant microbial populations were different at different temperatures.

关 键 词：垃圾处理 沼气 厌氧消化 微生物 细菌 物种多样性 

分 类 号：TK6[动力工程及工程热物理—生物能]