江苏省南通市疟疾诊断参比实验室病例复核结果分析  

Analysis of malaria cases re-examination results of malaria diagnostic reference laboratory in Nantong, Jiangsu

作　　者：曹彩群[1] 丁桂生[1] 卢金 顾亚萍[2] CAO Cai-qun;DING Gui-sheng;LU Jin;GU Ya-ping(Nantong Municipal Center for Disease Control and Prevention,Nantong,Jiangsu 226006,China;National Health Commission Key Laboratory of Parasitic Disease Control and Prevention,Jiangsu Institute of Parasitic Diseases,Wuxi,Jiangsu 214064,China)

机构地区：[1]江苏省南通市疾病预防控制中心,江苏南通226006 [2]江苏省血吸虫病防治研究所,国家卫生健康委员会寄生虫病预防与控制技术重点实验室,江苏无锡214064

出　　处：《中国热带医学》2022年第9期870-873,共4页China Tropical Medicine

摘　　要：目的 分析南通市疟疾诊断参比实验室对2014—2021年国家传染病报告系统疟疾病例的实验室镜检复核结果,评价南通市疟疾诊断参比实验室疟疾诊断能力。方法 收集南通市2014—2021年国家传染病报告系统的疟疾病例的血涂片和全血样本,南通市与江苏省血吸虫病防治研究所疟疾诊断参比实验室开展市、省两级实验室复核,以省级疟疾诊断参比实验室镜检和核酸检测(PCR)复核结果一致为标准,比较分析南通市疟疾诊断参比实验室复核结果。结果 2014—2021年,南通市与江苏省两级实验室复核297例病例血涂片和血样。省级镜检和PCR复核结果一致,复核结果定性为292例阳性,5例阴性。南通市镜检复核结果与省级复核结果定性符合率100%(297/297),无错判和漏判;阳性血片虫种符合率96.23%(281/292),恶性疟原虫、间日疟原虫、卵形疟原虫和三日疟原虫符合率分别为99.57%(234/235)、62.50%(5/8)、89.47%(34/38)、72.73%(8/11)。经一致性检验,市、省两级实验室虫种分型Kappa值为0.89;恶性疟原虫、间日疟原虫、卵形疟原虫和三日疟原虫分型Kappa值分别为0.98、0.58、0.87和0.79。结论 南通市疟疾诊断参比实验室疟疾诊断能力总体良好,需提高对疟原虫分型能力。Objective To analyze the laboratory microscopic re-examination results of malaria cases in Nantong of the National Notifiable Disease Report System from 2014 to 2021 by Nantong Malaria Diagnostic Reference Laboratory, so as to evaluate the malaria diagnosis ability of Nantong Malaria Diagnostic Reference Laboratory.Methods The blood smear and blood samples of malaria cases in Nantong from 2014 to 2021 of the National Notifiable Disease Report System were collected.Nantong Malaria Diagnostic Reference Laboratory and Jiangsu Institute of Parasitic Diseases carried out the re-examination of municipal and provincial laboratories, taking the results of provincial laboratory as the standard to compare and analyze the re-examination results of Nantong Malaria Diagnostic Reference Laboratory.Results From 2014 to 2021, the two-level laboratories in Nantong city and Jiangsu Province re-examined the blood samples of 297 malaria cases. The microscopic examination and PCR re-examination results at the provincial level were the same:292 positive cases and 5 negative cases.The qualitative coincidence rate between Nantong microscopic re-examination results and the provincial re-examination results was 100%(297/297), without misjudgment and omission. The coincidence rate of Plasmodium typing was 96.23%(281/292). The coincidence rate of P. falciparum, P. vivax, P. ovale and P. malaria were 99.57%(234/235), 62.50%(5/8), 89.47% (34/38) and 72.73%(8/11) respectively. The consistency test results showed that the Kappa value of Plasmodium typing results between municipal and provincial laboratories was 0.89. The Kappa values of P. falciparum, P. vivax,P. ovale and P. malaria were 0.98, 0.58, 0.87 and 0.79 respectively.Conclusion The malaria diagnosis ability of Nantong Malaria Diagnostic Reference Laboratory is generally good, and it is necessary to improve the ability of Plasmodium typing.

关 键 词：疟疾 参比实验室 复核 诊断能力 

分 类 号：R254.5[医药卫生—中医内科学]