PGT患者可活检囊胚数影响因素分析  

Analysis of Influencing Factors on the Number of Biopsiable Blastocyst in Preimplantation Genetic Testing Patients

作　　者：刘美 胡玥玥 刁红录 张昌军 LIU Mei;HU Yue-yue;DIAO Hong-lu;ZHANG Chang-jun(Reproductive Medicine Center,Renmin Hospital,Hubei University of Medicine;Hubei Clinical Research Center for Reproductive Medicine;Biomedical En-gineering College,Hubei University of Medicine;Biomedical Research Institute,Hubei University of Medicine;Hubei Key Laboratory of Embryonic Stem Cell Research,Hubei University of Medicine,Shiyan,Hubei 442000,China)

机构地区：[1]十堰市人民医院·湖北医药学院附属人民医院生殖医学中心 [2]湖北省生殖医学临床医学研究中心 [3]湖北医药学院生物医学工程学院 [4]湖北医药学院生物医药研究院 [5]胚胎干细胞研究湖北省重点实验室,湖北十堰442000

出　　处：《湖北医药学院学报》2022年第5期453-457,共5页Journal of Hubei University of Medicine

基　　金：湖北省自然科学基金创新群体(2020CFA021)。

摘　　要：目的:探讨影响行植入前胚胎基因检测技术(preimplantation genetic testing,PGT)患者的可活检囊胚数的取卵前临床因素。方法:回顾性分析166例采用PGT技术助孕患者的临床资料,通过单因素分析和多元线性回归分析筛选影响PGT患者可活检囊胚形成数的取卵前临床因素。结果:在单因素分析中发现,可活检囊胚形成数与不同染色体异常类型、体质指数(BMI)、扳机日孕酮水平、扳机日黄体生成素(LH)水平、促性腺激素(Gn)总量、取精当天精液的体积、浓度和前向运动精子比率(PR)均无显著相关(P>0.05);与女方年龄、血清抗缪勒管激素(AMH)水平、Gn天数、扳机日血清雌二醇水平及促排卵方案显著相关(P<0.05);多元线性回归分析提示在女方年龄、血清AMH水平、Gn天数、扳机日血清雌二醇水平及促排卵方案中,只有扳机日血清雌二醇水平是PGT患者可活检囊胚形成数的独立影响因素(回归系数为0.032,P<0.001)。结论:在PGT患者的可活检囊胚数的取卵前各临床因素中,只有扳机日血清雌二醇水平是PGT患者可活检囊胚形成数的独立影响因素。而女方年龄、AMH水平、Gn天数、促排卵方案均不是可活检囊胚形成数的独立影响因素。夫妻双方染色体核型异常与否不会影响可活检囊胚形成数。Objective To investigate the clinical factors affecting the number of biopsiable blastocysts in patients undergoing preimplantation genetic testing(PGT)before oocyte retrieval.Methods The clinical data of 166 patients with PGT assisted pregnancy were analyzed retrospectively.The clinical factors affecting the number of biopsiable blastocysts in PGT patients before oocyte retrieval were screened by single factor analysis and multiple linear regression analysis.Results In univariate analysis,there was no significant correlation found between the number of biopsiable blastocysts and the types of chromosomal abnormalities,body mass index(BMI),progesterone level on hCG day,luteinizing hormone(LH)level on HCG day,dose of gonadotropin(Gn)used,semen volume,concentration and progressive percentage(PR)on the day of sperm collection(P>0.05).However,the female’s age,AMH level,duration of Gn,estradiol level on HCG day and ovarian stimulation protocols were found significantly correlated with the number of biopsiable blastocysts(P<0.05).Multiple linear regression analysis showed that among female’s age,AMH,duration of Gn,serum estradiol on HCG days and ovarian stimulation protocols,only estradiol level on HCG days was an independent factor affecting the number of biopsiable blastocyst in PGT patients(regression coefficient=0.032,P<0.001).Conclusion Among the clinical factors of the number of biopsiable blastocysts in PGT patients before oocyte retrieval,only the serum estradiol level on the trigger day was an independent factor affecting the number of biopsiable blastocysts in PGT patients.However,female’s age,AMH,duration of Gn and ovarian stimulation protocols were not independent influencing factors for the number of biopsiable blastocysts.The number of biopsiable blastocyst was not affected by the abnormal karyotype of both spouses.

关 键 词：植入前胚胎基因检测技术 扳机日雌二醇水平 可活检囊胚形成数 

分 类 号：R714.8[医药卫生—妇产科学]