利用SSR分子标记鉴定华南型黄瓜‘川绿15号’杂交种子纯度  

Identification of Hybrid Seed Purity on south China Type Cucumber’Chuanlv15’ Using SSR Molecular Markers

作　　者：李春 梁根云[1,2] 蔡鹏 李跃建[1,2] 房超 刘独臣[1,2] 刘小俊 LI Chun;LIANG Genyun;CAI Peng;LI Yuejian;FANG Chao;LIU Duchen;LIU Xiaojun(Horticultural Institute,Sichuan Academy of Agricultural Sciences/Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province,Chengdu,Sichuan 610066,China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Southwest Region),Ministry of Agriculture and Rural Affairs,Chengdu,Sichuan 610066,China)

机构地区：[1]四川省农业科学院园艺研究所蔬菜种质与品种创新四川省重点实验室,四川成都610066 [2]农业农村部西南地区园艺作物生物学与种质创制重点实验室,四川成都610066

出　　处：《西昌学院学报（自然科学版）》2022年第3期13-17,共5页Journal of Xichang University(Natural Science Edition)

基　　金：国家现代农业产业技术体系专项资金项目(CARS-23-G38);四川省自然科学基金项目(2022NSFSC0177);国家现代农业产业技术体系四川蔬菜创新团队(川农函[2019]427号);四川省蔬菜育种攻关项目(2021YFYZ0022);四川省科技计划项目(2022YFYZ0004)。

摘　　要：[目的]为了快速、简便和准确地鉴定华南型黄瓜‘川绿15号’杂交种子的纯度,[方法]选用240个均匀分布在黄瓜基因组上的SSR分子标记对‘川绿15号’及其亲本进行多态性筛选及纯度鉴定。[结果]本研究从240个黄瓜SSR分子标记筛选到2对SSR标记(Cs100和Cs109)符合父母本间呈互补带型的要求,可用于川绿15号’杂交种子的纯度鉴定。准确性验证结果显示Cs100和Cs109的分子鉴定结果与田间形态的鉴定结果一致并且均能将掺入的其他品种黄瓜种子区分开来。[结论]本研究构建了以黄瓜特异性SSR分子标记Cs100和Cs109为核心引物的‘川绿15号’杂交种子纯度鉴定的技术方法,可快速、简便和准确地鉴定该品种的种子纯度,为该品种的制种和大面积推广提供技术支持。[Objective]To identify seed purity of south China type cucumber’ Chuanlv15’ quickly,simply and accurately.[Method]240 SSR molecular markers evenly distributing in cucumber genome were screened for polymorphism and purity identification.[Result]Two SSR molecular markers(Cs100 and Cs109)showed diversity between parents and were selected to identify the seeds purity of ’Chuanlv15’. The identification result using SSR markers was consist with the results from morphology in the field,and the two SSR markers could separate the ’Chuanlv15’ seeds from other varieties of seeds.[Conclusion]In this study,we established a quick,easy and accurate technical method to identify the seed purity of ’Chuanlv15’ with two specific SSR molecular markers(Cs100 and Cs109),which could supply the technical support for the seed commercialization and large extension of ’Chuanlv15’.

关 键 词：SSR标记 黄瓜 ‘川绿15号’ 纯度鉴定 

分 类 号：S642.2[农业科学—蔬菜学]