苯乙双胍对甲状腺癌细胞凋亡和侵袭的影响  

Effect of phenformin on apoptosis and invasion of thyroid cancer cells

作　　者：衣鲁江 潘世扬 YI Lu-jiang;PAN Shi-yang(Department of Laboratory Medicine,The First Affiliated Hospital of Nanjing Medical University/Jiangsu Provincial People’s Hospital,Nanjing 210000,Jiangsu Province,China)

机构地区：[1]南京医科大学第一附属医院、江苏省人民医院检验学部,江苏南京210000

出　　处：《中国临床药理学杂志》2022年第19期2301-2305,共5页The Chinese Journal of Clinical Pharmacology

基　　金：江苏省卫计委地区基金资助项目(ZDXKB2016005)。

摘　　要：目的探究苯乙双胍对甲状腺癌(thyroid cancer,TC)细胞凋亡和侵袭的干预作用。方法用0~20 nmol·L^(-1)苯乙双胍处理甲状腺上皮细胞系TEC和TC细胞系FTC133和TPC-1,用四参数逻辑曲线回归法测定半抑制浓度(half-inhibitory concentration,IC_(50))。将TEC细胞、TPC-1细胞和FTC133细胞均分为对照组和实验组;对照组均给予普通杜氏改良Eagle培养基进行常规培养,实验组分别给予含20,8和8 nmol·L^(-1)苯乙双胍的杜氏改良Eagle培养基进行培养。用流式细胞术检测细胞凋亡水平,用Transwell法检测细胞侵袭能力。结果苯乙双胍在TEC细胞、FTC133细胞和TPC-1细胞中的IC_(50)值分别为19.08,7.45和9.00 nmol·L^(-1)。经苯乙双胍干预24 h后,TEC细胞中实验组和对照组的凋亡率分别为(1.08±0.54)%和(0.70±0.24)%,FTC133细胞中实验组和对照组的凋亡率分别为(11.21±1.34)%和(5.22±0.79)%,TPC-1细胞中实验组和对照组的凋亡率分别为(12.80±0.57)%和(4.30±0.69)%。经苯乙双胍干预48 h后,FTC133细胞中实验组和对照组的侵袭细胞数分别为(58.33±7.77)和(95.33±11.50)个,TPC-1细胞中实验组和对照组的侵袭细胞数分别为(62.00±11.53)和(100.33±14.57)个。经苯乙双胍处理后,FTC133细胞和TPC-1细胞的凋亡率均显著升高,细胞侵袭能力均显著降低,差异均有统计学意义(均P<0.05),但对TEC细胞的凋亡未造成显著影响(P>0.05)。结论苯乙双胍对甲状腺癌细胞恶性生物学行为具有抑制作用,但对正常甲状腺细胞活性无显著影响。Objective To investigate the interventional effects of phenformin on apoptosis and invasion of thyroid cancer(TC)cells.Methods The thyroid epithelial cell line TEC and thyroid cancer cell lines FTC133 and TPC-1 were treated with 0-20 nmol·L^(-1) phenformin.The four-parameter logistic curve regression method determined the half-inhibitory concentration(IC_(50)).TEC cells,TPC-1 cells and FTC133 cells were divided into control and experimental groups.The control groups were given DMEM medium for traditional culture,while the experimental groups were given DMEM medium containing 20,8 and 8 nmol·L^(-1) phenformin,respectively.Flow cytometry was used to detect the level of cell apoptosis,and the Transwell method was used to detect cell invasion ability.Results The IC_(50) values of phenelzine in TEC cells,FTC133 cells and TPC-1 cells were 19.08,7.45 and 9.00 nmol·L^(-1),respectively.After 24 hours of intervention with phenformin,the apoptosis rates of the experimental and control groups in TEC cells were(1.08±0.54)%and(0.70±0.24)%,the apoptosis rates of the experimental and control groups in FTC133 cells were(11.21±1.34)%and(5.22±0.79)%,the apoptosis rates of the experimental and control groups in TPC-1 cells were(12.80±0.57)%and(4.30±0.69)%,respectively.After 48 hours of intervention with phenformin,the number of invasive cells in the experimental and control groups in FTC133 cells was(58.33±7.77)and(95.33±11.50),the number of invasive cells in the experimental and control groups in TPC-1 cells was(62.00±11.53)and(100.33±14.57).After treatment with phenformin,the apoptosis rates of FTC133 cells and TPC-1 cells were significantly increased,and the cell invasion abilities were significantly reduced.The differences were statistically significant(all P<0.05),but neither had a significant impact on the apoptosis of TEC cells(P>0.05).Conclusion Phenformin has an inhibitory effect on the malignant biological behaviour of thyroid cancer cells but without a significant effect on normal thyroid cell activity.

关 键 词：苯乙双胍 甲状腺癌 凋亡 侵袭 

分 类 号：R979.1[医药卫生—药品]