高效液相色谱-质谱联用法测定人全血中硼替佐米浓度  

Determination of bortezomib in human whole blood by HPLC-MS/MS

作　　者：韩菲菲[1] 朱莹[1] 刘洪川[1] 李鹏飞[1] 胡婷 赵瑞[1] 杜萍[1] 安卓玲[1] HAN Fei-fei;ZHU Ying;LIU Hong-chuan;LI Peng-fei;HU Ting;ZHAO Rui;DU Ping;AN Zhuo-ling(Department of Pharmacy,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China)

机构地区：[1]首都医科大学附属北京朝阳医院药事部,北京100020

出　　处：《中国临床药理学杂志》2022年第19期2334-2337,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的建立一种测定人全血硼替佐米血药浓度的方法。方法以ZnSO_(4)溶液破碎细胞,乙腈为沉淀剂沉淀蛋白,用高效液相串联质谱系统(HPLC-MS/MS)分析,色谱柱:Waters X Bridge C_(18)(2.1 mm×50.0 mm,3.5μm),流动相:甲醇(含0.1%甲酸)-水(含0.1%甲酸),流速:0.4 mL·min^(-1),柱温:40℃,进样量:2μL,电喷雾电离,用多反应监测模式,定量分析离子对分别为m/z 367.2→225.9(硼替佐米)、m/z 237.1→194.2(内标卡马西平)。结果以卡马西平为内标标准曲线方程为y=7.83×10^(-3) x+1.77×10^(-3)(r=0.9972)。线性范围为2.00~300.00μg·mL^(-1)。批间和批内RSD在5.21%~10.00%,平均提取回收率均在101.38%~108.00%,稳定性好。结论本方法样品处理简便,灵敏度高,专属性强,适用于硼替佐米的全血血药浓度监测。Objective To establish a method for determination of bortezomib concentration in human whole blood.Methods The cells were broken in ZnSO_(4) solution,and the proteins were precipitated by acetonitrile and then were determined by high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS).The column was Waters XBrige C_(18)(2.1 mm×50.0 mm,3.5μm),and the mobile phase was methanol(0.1%formic acid)-water(0.1%formic acid.The flow rate:0.4 mL·min^(-1),column temperature:40℃,injection volume:2μL,electrospray ionization,multiple reaction monitoring mode,quantitative analysis of ion pair m/z 367.2→225.9(bortezomib),m/z 237.1→194.2(internal standard carbamazepine).Results The standard curve equation with carbamazepine as the internal standard was y=7.83×10^(-3) x+1.77×10^(-3)(r=0.9972).The linear range was 2.00-300.00μg·mL^(-1).The intra-and inter-batch RSD was 5.21%-10.00%,and the average recovery was 101.38%-108.00%.Conclusion The method is simple,sensitive and specific,and suitable for whole-blood concentration monitoring of bortezomib.

关 键 词：硼替佐米 高效液相色谱-质谱联用法 血药浓度 治疗药物监测 

分 类 号：R979.1[医药卫生—药品]