Box-Behnken响应面法优化大黄泻浊颗粒提取工艺  

Optimization of Extraction Process of Dahuang Xiezhuo Granules by Box-Behnken Re⁃sponse Surface Methodology

作　　者：王晶晶 程媛 忻晓东 唐贺[2] 关胜江[2] 李春花 WANG Jing-jing;CHENG Yuan;XIN Xiao-dong;TANG He;GUAN Sheng-jiang;LI Chun-hua(College of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Hebei Provin-cial Hospital of Traditional Chinese Medicine,Shijiazhuang 050011,China;Research and Development Center of TCM Formulation and Application Technology,Hebei University,Shijiazhuang 050090,China;Hebei Traditional Chinese Medicine Formulation Technology Innovation Center,Shijiazhuang 050090,China;Hebei Industrial Technology Institute for Traditional Chinese Medicine Preparation,Shijiazhuang 050000,China)

机构地区：[1]河北中医学院药学院,河北石家庄050200 [2]河北省中医院,河北石家庄050011 [3]河北省高校中药组方制剂应用技术研发中心,河北石家庄050090 [4]河北省中药组方制剂技术创新中心,河北石家庄050090 [5]河北省中药制剂产业技术研究院,河北石家庄050000

出　　处：《时珍国医国药》2022年第8期1886-1889,共4页Lishizhen Medicine and Materia Medica Research

基　　金：河北省重点研发计划项目(19272502D)。

摘　　要：目的优化大黄泻浊颗粒的提取工艺。方法在单因素试验的基础上,以加水量、提取时间、浸泡时间为考察因素,以大黄酸、阿魏酸、丹酚酸B含量及干膏率为考察指标,利用Box-Behnken响应面法设计实验,信息熵理论确定各指标权重,计算综合评分M;通过分析回归方程模型,考察各因素对提取效果的影响,优选出最佳提取工艺,并对该工艺进行验证。结果最佳提取工艺参数为加9倍量水,浸泡50 min,共提取222 min;验证试验结果显示,实际M值与模型预测M值相差<1.0%。结论优化的提取工艺稳定可靠,可为大黄泻浊颗粒后期开发和应用提供依据,也为医疗机构中药制剂研究提供参考。Objective To optimize the extraction process of Dahuang Xiezhuo granules.Methods On the basis of single factor experiment,with water addition,extraction time,soaking time as investigation factors,rhein,ferulic acid,salvianolic acid B content and dry extract rate as investigation indexes,the experiment was designed by Box-Behnken response surface method,the weight of each index was determined by information entropy theory,and the comprehensive score M was calculated.By analyzing the regression equation model,the influence of various factors on the extraction effect was investigated,and the best extraction process was selected and verified.Results The optimum extraction process parameters were as follows:adding 9 times of water,soaking for 50 min,and extracting for 222 min;The test results show that the difference between the actual M value and the pre⁃dicted M value of the model is less than 1.0%.Conclusion The optimized extraction process is stable and reliable,which can provide a basis for the later development and application of Dahuang Xiezhuo Granule,and also provide a reference for the re⁃search of traditional Chinese medicine preparations in medical institutions.

关 键 词：大黄泻浊颗粒 提取工艺 响应面法 信息熵 综合评分 

分 类 号：R283.6[医药卫生—中药学]