UPLC-MS/MS对肾阳虚原发性骨质疏松临床代谢标志物筛查研究  

Metabolomics analysis based on UPLC-MS/MS in primary osteoporosis with Kidney-Yang deficiency syndrome

作　　者：郭胜男[1] 戚晓楠 姚啸生[2] 任路[1] 陈文娜[1] GUO Shengnan;QI Xiaonan;YAO Xiaosheng;REN Lu;CHEN Wenna(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;The First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110000,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110000

出　　处：《中国骨质疏松杂志》2022年第10期1410-1415,共6页Chinese Journal of Osteoporosis

基　　金：国家重点研发计划中医药现代化研究重点专项(2018YFC1704301)。

摘　　要：目的 基于UPLC-MS/MS法筛查肾阳虚原发性骨质疏松症患者的临床差异代谢物,探讨肾阳虚的潜在分子机制。方法 收集肾阳虚家系中20例原发性骨质疏松症患者和12例健康人群的血清,运用UPLC-MS/MS法检出代谢物,并进行差异分析和信号通路的富集分析。结果 分析得到157个差异性代谢物,其中γ-氨基丁酸、L-异亮氨酸、黄嘌呤、十二烷二酸等93个代谢物上调,谷氨酸、丙氨酸、天冬氨酸、肌氨酸、苯乙酰甘氨酸等64个代谢物下调。差异代谢物参与的信号通路主要涉及色氨酸代谢、谷胱甘肽代谢、磷脂酶D信号通路、精氨酸和脯氨酸代谢、丙氨酸代谢等。结论 筛选出的差异代谢标志物可进一步用于肾阳虚骨质疏松症的分子机制研究,为研究中医肾阳虚证候的生物学本质提供了一定的理论依据。Objective To explore the potential mechanisms of Kidney-Yang deficiency syndrome(KYD)in primary osteoporosis, this study aims to analyze the plasma metabolomics. Methods Plasma samples were collected from 20 participants with primary osteoporosis of KYD and 12 participants from control of normal group.UPLC-MS/MS was used to detecte the samples.Then screened the differential metabolites and the related metabolic pathways. Results Total of 157 metabolites were identified. 93 metabolites were discovered increased include γ-aminobutyric acid, L-isoleucine, xanthine, dodecanedioic acid,while 64 metabolites were found declined include glutamic acid, alanine, aspartic acid, sarcosine, phenylacetylglycine. The mechanism mainly involved tryptophan metabolism, glutathione metabolism, phospholipase D signaling pathway and arginine, proline with alanine metabolism. Conclusion The metabolism markers can be further used to study the mechanism of primary osteoporosis of KYD. It provides the substantial basis for studying the biological essence of KYD in TCM.

关 键 词：代谢组学分析 肾阳虚 原发性骨质疏松症 

分 类 号：R25[医药卫生—中医内科学]