金天格胶囊对H_(2)O_(2)诱导的小鼠成骨细胞MC3T3-E1氧化应激损伤及炎症因子的作用  

Effect of Jintiange Capsule on osteoblast MC3T3-E1 induced by H_(2)O_(2) through inhibiting oxidative stress damage and the release of inflammatory cytokines in vitro

作　　者：李超 赵剑波 陈俊雅 耿玲 何宁 赵浩东 LI Chao;ZHAO Jianbo;CHEN Junya;GENG Ling;HE Ning;ZHAO Haodong(Traumatic Orthopedics,First Affiliated Hospital of Dali University,Dali 671000,China;Dali University,Dali 671000,China)

机构地区：[1]大理大学第一附属医院创伤骨科,云南大理671000 [2]大理大学,云南大理671000

出　　处：《中国骨质疏松杂志》2022年第10期1448-1452,1532,共6页Chinese Journal of Osteoporosis

基　　金：云南省教育厅科学研究基金项目(2020J0574)。

摘　　要：目的研究金天格胶囊对骨质疏松中氧化应激损伤和炎症因子释放的改善作用。方法体外培养MC3T3-E1小鼠成骨细胞,分别加入10、20、50和100μg/mL金天格胶囊溶液,测定药物作用不同时间后MC3T3细胞存活率,和细胞培养上清液中ALP和PICP含量。利用1 mmol/L H_(2)O_(2)建立MC3T3细胞诱导损伤模型,给予不同浓度金天格胶囊,测定细胞培养上清液中SOD、GSH、CAT和MDA含量和TNF-α、IL-6及IL-1β水平,然后测定MC3T3细胞中Tnfa、Il-6、Il-1b mRNA相对表达。结果与空白组比较,100μg/mL金天格胶囊能显著提高MC3T3-E1细胞存活率,50、100μg/mL金天格胶囊能显著增加细胞培养上清中ALP和PICP含量。在H_(2)O_(2)刺激MC3T3-E1细胞损伤模型中,50、100μg/mL金天格胶囊能显著提高H_(2)O_(2)诱导损伤后细胞存活率,并能明显增加细胞培养上清液中SOD、GSH、CAT活力,降低MDA水平;此外,与H_(2)O_(2)组相比,50、100μg/mL金天格胶囊能够显著降低MC3T3细胞培养上清中TNF-α、IL-6含量,显著降低细胞中Tnfa、Il-6 mRNA相对表达,100μg/mL金天格胶囊能降低Il-1b mRNA相对表达。结论金天格胶囊能促进小鼠成骨细胞MC3T3-E1增殖,并抑制由H_(2)O_(2)引起的炎症基因表达和炎症因子释放。Objective To study the amiliorative effect of Jintiange Capsule on osteoporosis by inhibiting oxidative stress injury and inflammatory cytokines release.Methods Mouse osteoblast MC3T3-E1 was cultured and incubated by 10,20,50 and 100μg/mL Jintiange Capsule solution.Then MC3T3-E1 cell viability and contents of ALP and PICP in cultural supernatant were determined.1 mmol/L H_(2)O_(2) was used to induce MC3T3-E1 cell injury model.The injury MC3T3-E1 cell was treated by different concentrations of Jintiange Capsule.Then the contents of oxidative factor MDA and anti-oxidative factors SOD,GSH-Px and CAT and cytokines TNF-α,IL-6 and IL-1βin cultural supernatant were assayed.The relative mRNA expression of Tnfa,Il6 and Il1b in MC3T3-E1 was determined.Results Compared with control group,100μg/mL Jintiange Capsule could improve cell viability of MC3T3-E1,both 50 and 100μg/mL Jintiange Capsule could increase contents of ALP and PICP in cultural supertanant.In the MC3T3-E1 cell injury model induced by H_(2)O_(2)50 and 100μg/mL Jintiange Capsule could increase cell viability and improve the activities of SOD,GSH-Px and CAT,decrease level of MDA.Compared with H_(2)O_(2) group,50 and 100μg/mL Jintiange Capsule could reduce contents of TNF-αand IL-6 in cultural supertanant and lower relative mRNA expression of Tnfa and Il6,100μg/mL Jintiange Capsule could decrase relative mRNA expression of Il1b.Conclusion Jintiange Capsule could improve cell proliferation and inhibit inflammatory gene expression and inflammatory cytokines release in MC3T3-E1 induced by H_(2)O_(2).

关 键 词：骨质疏松 金天格胶囊 成骨细胞 碱性磷脂酶 Ⅰ型前胶原羧基端前肽 

分 类 号：R965[医药卫生—药理学]