基因表达谱芯片分析续苓健骨方治疗去卵巢大鼠骨质疏松症的基因表达差异  

The diffe re nce of ge ne e xpre ssion of Xuling Jiangu Pre scription in tre ating ovarie ctomize d rats with oste oporosis was analyze d base d on ge ne e xpre ssion profile chip

作　　者：黄景文 李生强 陈玄 谢丽华 陈赛楠 黄小彬 葛继荣 HUANG Jingwen;LI Shengqiang;CHEN Xuan;XIE Lihua;CHEN Sainan;HUANG Xiaobin;GE Jirong(Fujian College of Traditional Chinese Medicine,Fuzhou 350001,China)

机构地区：[1]福建省中医药科学院,福建福州350001

出　　处：《中国骨质疏松杂志》2022年第10期1453-1458,共6页Chinese Journal of Osteoporosis

基　　金：福建省科技厅省属公益类科研院所基本科研专项项目(2019R1003-6)。

摘　　要：目的基因表达谱芯片筛选续苓健骨方治疗骨质疏松症模型大鼠的差异表达基因。方法将15只大鼠按随机数法分为3组:假手术组、模型组、续苓健骨方组,每组5只。去卵巢造模后1周开始灌胃给药,实验组按照体重给予续苓健骨方,另外两组灌胃与实验组等体积的生理盐水,给药连续13周,每日1次。13周后取大鼠左腿股骨进行基因表达谱芯片检测,分析芯片得到组间差异表达基因并进行功能和通路分析。结果以丨log2 Fold change丨≥1,q<0.05为条件筛选出10个造模后表达显著降低但用药后显著提高的基因,218个造模后表达显著提高但用药后表达显著降低的基因。KEGG信号通路富集分析发现PI3K/Akt通路是关键作用通路之一。设置条件(丨log2 Fold change丨≥2,q<0.01)进一步筛选获得差异基因Rplp0和Cnpy2。结论续苓健骨方的治疗作用机制可能与PI3K/AKT信号通路及Rplp0、Cnpy2基因相关。Objective To screen the differentially expressed genes of Xuling Jiangu Prescription in osteoporosis model rats by gene expression profile chip.Methods 15 rats were divided into three groups according to random number method:sham operation group,model group and Xuling Jiangu Fang group,with 5 rats in each group.The experimental group was given Xuling Jiangu Prescription according to body weight,and the other two groups were given the same volume of normal saline by intragastric administration,once a day,for 13 consecutive weeks.After 13 weeks,the femur of the left leg of the rat was taken for gene expression profile microarray detection,and the differentially expressed genes between groups were obtained by microarray analysis,and their functions and pathways were analyzed.Results Ten genes with significantly decreased expression after modeling but significantly increased expression after medication were screened,and 218 genes with significantly increased expression after modeling but significantly decreased expression after medication were screened under the conditions of (|log2 Fold change|≥1,Q<0.05).Enrichment analysis of KEGG signaling pathway revealed that PI3K/Akt pathway was one of the key pathways.The differential genes Rplp0 and Cnpy2 were further screened under the following conditions:(|log2 Fold change|≥2,q<0.01).Conclusion The therapeutic mechanism of Xuling Jiangu Prescription may be related to PI3K/AKT signaling pathway,Rplp0 and Cnpy2 genes.

关 键 词：中医中药 骨质疏松大鼠模型 基因表达谱芯片 差异表达基因 

分 类 号：R285.5[医药卫生—中药学] R332[医药卫生—中医学]