鸡冠花黄酮对骨质疏松大鼠TMAO/NF-κB/NFATc1水平的影响  

Effects of flavonoids from Cockscomb on TMAO/NF-κB/NFATc1 levels in rats with osteoporosis

作　　者：邓满香[1] 陈鹭玲[1] 何剑全[1] DENG Manxiang;CHEN Luling;HE Jianquan(Zhongshan Hospital affiliated to Xiamen University,Xiamen 361000,China)

机构地区：[1]厦门大学附属中山医院,福建厦门361000

出　　处：《中国骨质疏松杂志》2022年第10期1485-1490,共6页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金资助项目(82004433)。

摘　　要：目的探讨鸡冠花黄酮提取物对老年骨质疏松大鼠TMAO-NF-κB/NFATc1水平、骨微结构及骨特异性磷酸酶蛋白的作用机制。方法将16周龄大鼠分为假手术组(Sham组,n=13)、模型组(n=9)、干预组(n=11)及药物对照组(n=11),除Sham组外其余大鼠均建立OP模型,干预组灌胃鸡冠花黄酮提取物。结果与Sham组相比,模型组大鼠血清Ca、P、ALP及BAP水平均降低,差异具有统计学意义(P<0.05),与模型组相比,干预组大鼠血清Ca、P、ALP及BAP水平升高,差异具有统计学意义(P<0.05);Sham组、模型组、干预组及药物对照组的骨密度BMD分别为(58.05±6.11)mg/cm^(3)、(47.50±4.53)mg/cm^(3)、(55.17±5.14)mg/cm^(3)及(54.96±5.40)mg/cm^(3),组间比较差异具有统计学意义(F=6.983,P<0.001);与Sham组相比,模型组大鼠股骨组织中TMAO、NF-κB、NFATc1表达较高(P<0.05),与模型组相比,干预组大鼠组织中TMAO、NF-κB、NFATc1表达降低,差异具有统计学意义(P<0.05)。结论老年骨质疏松大鼠采用鸡冠花黄酮提取物干预后可显著改善骨微结构,提高骨特异性磷酸酶活性并增加骨密度,机制可能与抑制TMAO、NF-κB、NFATc1表达相关。Objective To investigate the effect of flavonoids extract of Cockscomb on tMAO-NF-κB/NFATc1 level,bone microstructure and bone specific phosphatase protein in aged osteoporosis rats.Methods 16-week-old rats were divided into sham operation group(SHAM group,n=13),model group(n=9),intervention group(n=11)and drug control group(n=11).OP model was established in all rats except sham group.Results Compared with sham group,serum LEVELS of Ca,P,ALP and BAP in model group were decreased,and there were differences(P<0.05);compared with model group,serum levels of Ca,P,ALP and BAP in intervention group were increased,and there were differences(P<0.05);BMD of sham group,model group,intervention group and drug control group were(58.05±6.11)mg/cm^(3),(47.50±4.53)mg/cm^(3),(55.17±5.14)mg/cm^(3) and(54.96±5.40)mg/cm^(3),and there were differences among groups(F=6.983,P<0.001).Compared with sham group,TMAO,NF-κB and NFATc1 expressions were higher in model group than in sham group(P<0.05),while TMAO,NF-κB and NFATc1 expressions were lower in intervention group than in model group(P<0.05).Conclusion The intervention of flavonoids extract of C.cristatum can significantly improve bone microstructure,increase bone-specific phosphatase activity and increase bone mineral density in aged rats with osteoporosis.The mechanism may be related to the inhibition of TMAO,NF-κB and NFATc1 expression.

关 键 词：骨质疏松 鸡冠花黄酮提取物 骨微结构 骨特异性磷酸酶 

分 类 号：R965[医药卫生—药理学]