载银/钴β-磷酸三钙的抗菌性能评估及其应用于感染性骨缺损治疗研究  

Ag and Co elements endow beta-tricalcium phosphate with theboosted bactericidal ability for bone defect contagion therapy

作　　者：何帅 刘昱辰 韩秋阳 汪寿腾 谢克难[1] 谢璐 邓怡[1] 杨为中[3] HE Shuai;LIU Yuchen;HAN Qiuyang;WANG Shouteng;XIE Kenan;XIE Lu;DENG Yi;YANG Weizhong(School of Chemical Engineering,Sichuan University,Chengdu 610065,China;PLA hospital of Unit 63820,Mianyang 621051,China;College of Biomedical Engineering,Sichuan University,Chengdu 610065,China;West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学化学工程学院,成都610065 [2]中国人民解放军63820部队医院,四川绵阳621051 [3]四川大学生物医学工程学院,成都610065 [4]四川大学华西口腔医院,成都610041

出　　处：《功能材料》2022年第10期10032-10038,共7页Journal of Functional Materials

基　　金：国家自然科学基金委员会与香港研究资助局联合科研资助基金项目(81961160736);国家自然科学基金委员会青年科学基金项目(81801848,52002255)。

摘　　要：由外伤、骨髓炎和骨肉瘤切除手术等引起的骨缺损问题正在全球范围内威胁着患者的健康。目前,植入骨修复材料以填充缺损部位是治疗骨缺损的主流方法。然而,由于传统的骨植入材料抗菌能力差,易受到病原体入侵,易发生植入体周围感染(IAI),从而导致手术失败并对患者造成继发性损伤。为解决这个问题,研究中通过压制成型及聚多巴胺辅助改性构建了由钴、银纳米颗粒和生物陶瓷β-磷酸三钙(β-TCP)组成的新型多功能植入物(Ag@Co/β-TCP)。由于Ag+和Co^(2+)的固有杀菌能力,Ag@Co/β-TCP植入体对革兰氏阳性(金黄色葡萄球菌)和革兰氏阴性(大肠杆菌)病原菌表现出优异的抗菌性能。此外,体外细胞实验表明Ag@Co/β-TCP具有良好的生物相容性和促细胞生长能力。这些优点可使功能化生物陶瓷Ag@Co/β-TCP在不使用抗生素的条件下达到抗菌和成骨的目的,以期解决植入手术过程中所引发的IAI问题。Bone defect caused by trauma,osteomyelitis,and osteosarcoma resection surgery is threatening the patients’health globally.Currently,implanting orthopedic materials is the mainstream method for repairing bone defects by filling bone defects.However,owing to the poor bactericidal ability,conventional orthopedic implant materials are susceptible to pathogen invasion leading to implant-associated infection(IAI),which would prolong the suffering of patients and cause secondary injury.To cope with this issue,herein,we construct a novel multifunctional implant(Ag@Co/β-TCP)consisting of cobalt metal,silver nanoparticles,and bioceramic beta-tricalcium phosphate(β-TCP)through compression shaping and polydopamine-assisted modification for the purpose of antibiotic-free IAI therapy.Owing to the intrinsic germicidal activity of Ag^(+)and Co^(2+),Ag@Co/β-TCP implants exhibit excellent anti-bacterial properties against Gram-positive(Staphylococcus aureus)and Gram-negative(Escherichia coli)pathogenic bacteria.Additionally,in vitro cell experiments demonstrate that Ag@Co/β-TCP displays acceptable biocompatibility and cell growth ability.Accordingly,these merits make the functionalized bioceramic become a promising antibiotic-free anti-bacterial bone implant material for combating the issue of IAI during the implantation process by boosting the anti-bacterial ability ofβ-TCP.

关 键 词：Β-磷酸三钙 生物陶瓷 银 抗感染 骨植入体相关感染 

分 类 号：TB34[一般工业技术—材料科学与工程]