和田羊Lin28B基因启动子区的克隆及序列分析  

Cloning and Sequence Analysis of the Promoter Region of Lin28B Gene in Hetian Sheep

作　　者：隋志远 张继虎 李庆瑾 张志帅 张永杰 邢凤[1] SUI Zhiyuan;ZHANG Jihu;LI Qingjin;ZHANG Zhishuai;ZHANG Yongjie;XING Feng(College of Animal Science and Technology,Tarim University/Key Laboratory Tarim Animal Husbandry Science and Technology Xinjiang Production&Construction Corps,Alar 843300,Xinjiang,China)

机构地区：[1]塔里木大学动物科学与技术学院/新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300

出　　处：《四川农业大学学报》2022年第5期649-655,共7页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金项目(31960655)。

摘　　要：【目的】分析和田羊Lin28B基因的启动子序列与结构,为进一步解析其转录调控机制奠定基础。【方法】根据Ensembl数据库中绵羊Lin28B基因5’侧翼区序列,设计PCR引物进行扩增、克隆测序及序列鉴定,得到和田羊Lin28B基因启动子区序列,并利用相关生物信息学软件对其进行预测分析。【结果】试验成功获得2 993 bp的和田羊Lin28B基因启动子区序列,GC含量为39.33%;预测了其核心启动子区域,发现在启动子区存在7个潜在的转录起始位点,同时发现了2个TATA-box、2个CpG岛,但未发现CAAT-box;该序列还包含AP-1、Oct-1、p53、Pax-4、STAT3、TGIF等转录因子结合位点。【结论】运用生物信息学软件预测分析和田羊Lin28B基因启动子序列,可为更深入研究Lin28B基因对绵羊初情期的调控机制提供理论基础。[Objective] The promoter sequence and structure of Lin28 B gene in Hetian sheep were analyzed to lay the foundation for further analysis of its transcriptional regulation mechanism.[Method] According to the 5’ flanking region sequence of sheep Lin28 B gene in Ensembl database,PCR primers were designed for amplification,cloning,sequencing and sequence identification to obtain the promoter region sequence of Lin28 B gene in Hetian sheep, and the relevant bioinformatics softwares were used to predict and analyze the promoter region sequence.[Result] The 2 993 bp sequence of Lin28 B gene promoter region of Hetian sheep was successfully obtained, and GC content was 39.33%.The core promoter region was predicted,and 7 potential transcription start sites were found in the promoter region.Besides, 2 TATA-boxes and2 CpG islands were found, but no CAAT-box was found.The sequence was also found to contain binding sites for transcription factors such as AP-1,Oct-1,p53,Pax-4,STAT3 and TGIF.[Conclusion] Bioinformatics softwares were used to predict and analyze the promoter sequence of Lin28 B gene in Hetian Sheep, which can provide a theoretical basis for further research on the regulation mechanism of Lin28 B gene on sheep puberty.

关 键 词：和田羊 Lin28B基因 启动子区 序列分析 结构预测 

分 类 号：S811.6[农业科学—畜牧学]