3种生长调节剂对长足大竹象幼虫的毒力及相关酶活性影响  

Effects of Three Growth Regulators on the Toxicity of Cyrtotrachelus buqueti and the Activities of Related Enzymes

作　　者：王帆 杨梅 陈章铭 杨桦[1] 杨伟[1] 黄琼[1] WANG Fan;YANG Mei;CHEN Zhangming;YANG Hua;YANG Wei;HUANG Qiong(College of Forestry,Sichuan Agricultural University,Chengdu 611130,China/Key Laboratory of Ecological Forestry Engineering of Sichuan Province,Chengdu 611130,China;Nanjiang County Forestry Bureau of Sichuan Province,Nanjiang 635600,Sichuan,China)

机构地区：[1]四川农业大学林学院/四川省林业生态工程省级重点实验室,成都611130 [2]四川省南江县林业局,四川南江635600

出　　处：《四川农业大学学报》2022年第5期775-781,共7页Journal of Sichuan Agricultural University

基　　金：四川省国际科技创新合作项目(2021YFH0160)。

摘　　要：【目的】为了研究甲氧虫酰肼、氟铃脲和吡丙醚,3种昆虫生长调节剂对长足大竹象Cyrtotrachelus buqueti幼虫的杀虫活性。【方法】采用饲喂法测定上述3种药剂对长足大竹象2龄和4龄幼虫的毒力、温度效应及对其体内相关酶活性的影响。【结果】3种昆虫生长调节剂对长足大竹象2龄和4龄幼虫的毒力以甲氧虫酰肼对2龄幼虫毒力最高,LC_(50)为28.13 mg/L;而吡丙醚对4龄幼虫毒力最高,LC_(50)为111.55 mg/L。在28℃下,氟铃脲和吡丙醚对长足大竹象4龄幼虫毒力最高,LC_(50)分别为294.11 mg/L和71.51 mg/L,但甲氧虫酰肼的毒力受温度的影响无规律性。3种生长调节剂对4龄幼虫体内的乙酰胆碱酯酶、谷胱甘肽-S-转移酶、羧酸酯酶、过氧化物酶和超氧化物歧化酶存在显著影响,表现为抑制作用;在不同的处理时间,对这些酶的影响存在明显差异。【结论】研究结果有助于深入了解长足大竹象对不同类型生长调节剂的防御机制,以及对该虫的综合治理和丛生竹安全生产提供理论和技术支持。[Objective] This paper aims to study the insecticidal activities of methoxytebufenazide,flubrillamide and pyriproxyfen on Cyrtotrachelus buqueti larva.[Method] Using the feeding method,this study determined the toxicity,temperature effect and influence on related enzyme activity in vivo of different instars of C.buqueti larva.[Result] The results showed that the toxicity of the three kinds of insect growth regulators to the 2 nd and 4 th instar larvae of C.buqueti was the highest,and LC_(50) was 28.13 mg/L.The toxicity of pyriproxyfen to the 4 th instar larva was the highest and LC_(50) was 111.55 mg/L.At28 ℃;the toxicity of flubrilluron and pyriproxyfen to the 4 th instar larva of C.buqueti was the highest,LC_(50) was 294.11 mg/L and 71.51 mg/L respectively, but the toxicity of methoxytezide was not regularly affected by temperature.Three kinds of growth regulators had significant effects on acetylcholin esterase,glutathione s-transferase, carboxylesterase,peroxidase and superoxide dismutase in the 4 th instar larva,which showed inhibition.The effects of different treatment time on these enzymes were significantly different.[Conclusion] The results of this study will be helpful for further understanding of the defense mechanism of C.buqueti against different types of growth regulators and provide theoretical and technical support for the comprehensive control of this insect and the safe production of sympodial bamboo.

关 键 词：长足大竹象 昆虫生长调节剂 毒力 酶活性 

分 类 号：Q965.9[生物学—昆虫学]