姜黄素对脂多糖诱导肺细胞损伤的作用及其机制研究  

Mechanism of curcumin in alleviating LPS-induced lung cell injury

作　　者：邹国涛[1] 曾毅文 钟绍文 潘云波 Guo-tao Zou;Yi-wen Zeng;Shao-wen Zhong;Yun-bo Pan(Department of Pediatrics,Yongchuan Hospital of Chongqing Medical University,Chongqing 402160,China)

机构地区：[1]重庆医科大学附属永川医院儿科,重庆402160

出　　处：《中国现代医学杂志》2022年第20期41-48,共8页China Journal of Modern Medicine

基　　金：重庆市教育委员会科学技术研究项目(No:KJQN201900420);重庆市科学基金项目(No:cstc2021jcyj-jqX0035)。

摘　　要：目的探究姜黄素通过介导长链非编码RNA THRIL(lncRNA THRIL)表达对脂多糖(LPS)诱导的人正常肺上皮细胞(BEAS-2B)损伤的影响。方法LPS诱导BEAS-2B细胞复制体外急性肺损伤细胞模型,并加入姜黄素处理。采用Lipofectamine?2000转染试剂将lncRNA THRIL过表达/敲降载体或空载体转染到BEAS-2B细胞中。通过实时荧光定量聚合酶链反应和酶联免疫吸附试验检测lncRNA THRIL及炎症细胞因子[白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)]水平;CCK-8法和流式细胞术分别检测LPS、姜黄素及THRIL对细胞活性和细胞凋亡的影响。结果姜黄素组与对照组lncRNA THRIL相对表达量比较,差异无统计学意义(P>0.05),LPS组较对照组升高(P<0.05),LPS+姜黄素2.5μmol/L组、LPS+姜黄素5μmol/L组、LPS+姜黄素7.5μmol/L组较LPS组降低(P<0.05)。姜黄素组与对照组细胞凋亡率比较,差异无统计学意义(P>0.05),LPS组较对照组升高(P<0.05),LPS+姜黄素2.5μmol/L组、LPS+姜黄素5μmol/L组、LPS+姜黄素7.5μmol/L组较LPS组降低(P<0.05)。姜黄素组与对照组IL-1β、IL-6和TNF-α相对表达量及浓度比较,差异无统计学意义(P>0.05),LPS组较对照组升高(P<0.05),LPS+姜黄素2.5μmol/L组、LPS+姜黄素5μmol/L组、LPS+姜黄素7.5μmol/L组较LPS组降低(P<0.05)。姜黄素组与对照组细胞活性比较,差异无统计学意义(P>0.05),LPS组较对照组降低(P<0.05),LPS+姜黄素2.5μmol/L组、LPS+姜黄素5μmol/L组、LPS+姜黄素7.5μmol/L组较LPS组升高(P<0.05)。敲降THRIL组lncRNA THRIL的相对表达量较敲降阴性对照组降低(P<0.05)。LPS+敲降阴性对照组与LPS组细胞凋亡率比较,差异无统计学意义(P>0.05),LPS组较对照组升高(P<0.05),LPS+敲降THRIL组较LPS+敲降阴性对照组降低(P<0.05)。LPS+敲降阴性对照组与LPS组IL-1β、IL-6和TNF-α的相对表达量及浓度比较,差异无统计学意义(P>0.05),LPS组较对照组升高(P<0.05),LPS+敲降THRIL组较LPS+敲降Objective To explore the effect of curcumin on lipopolysaccharide(LPS)-induced lung epithelial injury by mediating the expression of long non-coding RNA THRIL.Methods The BEAS-2B cells were subject to LPS to establish the acute lung epithelial injury cell models in vitro,and they were treated with curcumin.LncRNA THRIL overexpression/knockdown vectors or empty vectors were transfected into BEAS-2B cells via Lipofectamine2000.The expression levels of lncRNA THRIL and inflammatory cytokines(IL-1β,IL-6,and TNF-α)were detected by quantitative real-time polymerase chain reaction(qRT-PCR)or enzyme-linked immunosorbent assay(ELISA).The effects of LPS,curcumin and lncRNA THRIL on cell viability and apoptosis were measured by flow cytometry and CCK-8.Results There was no difference in the expression of lncRNA THRIL between the curcumin group and the control group(P>0.05).However,the expression of lncRNA THRIL was higher in the LPS group than in the control group(P<0.05),whereas it was lower in the LPS+2.5μmol/L curcumin group,LPS+5μmol/L curcumin group and LPS+7.5μmol/L curcumin group than in the LPS group(P<0.05).There was no difference in the levels of IL-1β,IL-6,and TNF-αbetween the curcumin group and the control group(P>0.05).In contrast,the levels of IL-1β,IL-6,and TNF-αwere higher in the LPS group than in the control group(P<0.05),while they were lower in the LPS+2.5μmol/L curcumin group,LPS+5μmol/L curcumin group and LPS+7.5μmol/L curcumin group than in the LPS group(P<0.05).The expression of lncRNA THRIL was lower in the THRIL knockdown group than that in the knockdown negative control group(P<0.05).There was no difference in the apoptosis rate between the LPS+knockdown negative control group and the LPS group(P>0.05),while the apoptosis rate was higher in the LPS group compared with the control group(P<0.05)and was lower in the LPS+THRIL knockdown group compared with the LPS+knockdown negative control group(P<0.05).The levels of IL-1β,IL-6,and TNF-αwere not different between the LPS+THRIL knockdown

关 键 词：急性肺损伤 姜黄素 长链非编码RNA 炎症 

分 类 号：R563.8[医药卫生—呼吸系统]