Contractility detection of isolated mouse papillary muscle using myotronic Myostation-Intact device  

作　　者：Hong Lian Zhuyun Qin Mengge Wu Peipei Zuo Lina Bai Minjie Lu Lulu Li Haitao Zhang 

机构地区：[1]State Key Laboratory of Cardiovascular Disease,Fuwai Hospital,National Center for Cardiovascular Disease,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China [2]Beijing Key Laboratory of Preclinical Research and Evaluation for Cardiovascular Implant Materials,Animal Experimental Center,Fuwai Hospital,National Center for Cardiovascular Disease,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China [3]Adult Surgical Intensive Care Unit,Fuwai Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China [4]National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine,Fuwai Central-China Hospital,Central-China Subcenter of National Center for Cardiovascular Diseases,Zhengzhou,China

出　　处：《Animal Models and Experimental Medicine》2022年第5期445-452,共8页动物模型与实验医学（英文）

基　　金：Specialized Project of Fuwai Hospital,Grant/Award Number:2022-FWTS07;Shenzhen Sanming Project of Medicine,Grant/Award Number:2016-SZZF02;National Natural Science Foundation of China,Grant/Award Number:81900343;CAMS Innovation Fund for Medical Sciences,Grant/Award Number:CIFMS,2021-I2M-C&T-A-011。

摘　　要：Background:To understand the relationship between myocardial contractility and ex-ternal stimuli,detecting ex vivo myocardial contractility is necessary.Methods:We elaborated a method for contractility detection of isolated C57 mouse papillary muscle using Myostation-Intact system under different frequencies,volt-ages,and calcium concentrations.Results:The results indicated that the basal contractility of the papillary muscle was 0.27±0.03 mN at 10 V,500-ms pulse duration,and 1 Hz.From 0.1 to 1.0 Hz,con-tractility decreased with an increase in frequency(0.45±0.11-0.10±0.02 mN).The voltage-initiated muscle contractility varied from 3 to 6 V,and the contractility gradu-ally increased as the voltage increased from 6 to 10 V(0.14±0.02-0.28±0.03 mN).Moreover,the muscle contractility increased when the calcium concentration was increased from 1.5 to 3 mM(0.45±0.17-1.11±0.05 mN);however,the contractility stopped increasing even when the concentration was increased to 7.5 mM(1.02±0.23 mN).Conclusions:Our method guaranteed the survivability of papillary muscle ex vivo and provided instructions for Myostation-Intact users for isolated muscle contractility investigations.

关 键 词：calcium concentration frequency isolated papillary muscle muscle contractility Myostation-intact VOLTAGE 

分 类 号：Q95-33[生物学—动物学]