miR-129-5p抑制糖尿病视网膜病变血管生成的实验研究  

Experimental study of miR-129-5p inhibiting angiogenesis in diabetic retinopathy

作　　者：侯银芬 查晓娟 吴昌凡 张鹏飞 王娟 季霞 谢平[1] HOU Yinfen;ZHA Xiaojuan;WU Changfan;ZHANG Pengfei;WANG Juan;JI Xia;XIE Ping(Department of Ophthalmology,The First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学第一附属医院眼科,江苏南京210029 [2]皖南医学院第一附属医院弋矶山医院健康管理中心,安徽芜湖241001 [3]皖南医学院第一附属医院弋矶山医院眼科,安徽芜湖241001

出　　处：《皖南医学院学报》2022年第5期413-415,419,共4页Journal of Wannan Medical College

基　　金：国家自然科学基金面上项目(82070979);皖南医学院中青年科研基金项目(WK2018F01)。

摘　　要：目的:探究miR-129-5p在糖尿病视网膜病变(DR)中的作用及调控机制。方法:采用实时荧光定量PCR检测miR-129-5p在人视网膜微血管内皮细胞模拟体内高糖环境中和构建的糖尿病大鼠中的表达。进行功能实验EdU和CCK8的测定以探索miR-129-5p在视网膜血管功能障碍中的作用。通过Matrigel管形成实验验证视网膜血管功能障碍中的作用。结果:在糖尿病老鼠模型和模拟高糖环境下miR-129-5p表达降低(P<0.01),糖尿病条件下miR-129-5p的上调抑制了人视网膜微血管内皮细胞的增殖、迁移和Matrigel管形成(P<0.01)。结论:miR-129-5p抑制DR的增殖和Matrigel管的形成,为DR提供了潜在的治疗策略。Objective:To investigate the role and regulatory mechanism of miR-129-5p in diabetic retinopathy.Methods:Real-time fluorescent quantitative PCR was used to detect the expression of miR-129-5p in human retinal microvascular endothelial cells in a simulated high glucose environment and in diabetic mice model developed.Functional experiment using EdU and CCK8 was performed to explore the role of miR-129-5p in retinal vascular dysfunction,of which was verified by Matrigel tube formation assay.Results:The expression of miR-129-5p was decreased in diabetic mouse models and simulated high glucose environment(P<0.01),and up-regulation of miR-129-5p under diabetic condition inhibited the proliferation,migration and Matrigel tube formation of human retinal microvascular endothelial cells(P<0.01).Conclusion:miR-129-5p can inhibit the proliferation of diabetic retinopathy and the formation of Matrigel tubes.The findings may provide a potential treatment strategy for diabetic retinopathy.

关 键 词：糖尿病视网膜病变 血管生成 增殖 miR-129-5p 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]