琯溪蜜柚黄酮重结晶工艺优化  

Optimization of recrystallization process of flavonoids from Guanxi pomelo

作　　者：胡阳[1,2,3] 郑丽燕 杨远帆 吴锦辉[4] 倪辉 HU Yang;ZHENG Li-yan;YANG Yuan-fan;WU Jin-hui;NI Hui(College of Ocean Food and Biological Engineering,Jimei University,Xiamen,Fujian 361021,China;Key Laboratory of Food Microbiology and Enzyme Engineering Technology of Fujian Province,Xiamen,Fujian 361021,China;Research Center of Food Biotechnology of Xiamen City,Xiamen,Fujian 361021,China;Zhangzhou King Pemole Skin Biotechnology Co.,Ltd.,Zhangzhou,Fujian 363000,China)

机构地区：[1]集美大学海洋食品与生物工程学院,福建厦门361021 [2]福建省食品微生物与酶工程重点实验室,福建厦门361021 [3]厦门市食品生物工程技术研究中心,福建厦门361021 [4]漳州柚皮王生物技术有限公司,福建漳州363000

出　　处：《食品与机械》2022年第10期158-163,共6页Food and Machinery

基　　金：福建省科技厅引导性项目(编号:2021N0015);福建省平和县现代农业产业园中央财政奖补资金项目(编号:PH2020)。

摘　　要：目的:提高黄酮重结晶得率和纯度。方法:以超声波辅助乙醇提取法提取的蜜柚柚皮黄酮粗品为原料,利用响应面分析法优化乙醇重结晶纯化黄酮的工艺参数。结果:影响黄酮重结晶得率的主要因素有乙醇体积分数、结晶时间、pH以及乙醇体积分数和pH的交互作用,其中乙醇体积分数的影响最为显著。结论:黄酮重结晶的最优工艺条件为乙醇体积分数24%、液料比10∶1(mL/g)、pH 3.75、结晶温度4℃和结晶时间24 h,黄酮结晶得率为8.76%,结晶产物主要为柚皮苷,纯度为83.38%。Objective:This study aimed to improve the recrystallization yield and purity of flavonoid.Methods:Taking the crude flavonoids from pomelo peel extracted by ultrasonic-assisted ethanol extraction as raw materials,the process parameters of ethanol recrystallization and purification of flavonoids were optimized by response surface methodology.Results:The main factors affecting the recrystallization yield of flavonoids were ethanol concentration,crystallization time,pH value,and the interaction between ethanol concentration and pH value,among which,the ethanol concentration affacted most significantly.Conclusion:The optimal recrystallization conditions of flavonoids were as follows:ethanol volume fraction of 24%,liquid-solid ratio of 10∶1(mL/g),pH 3.75,crystallization temperature of 4℃,and crystallization time of 24 h.The crystallization yield of flavonoids was 8.76%,and the main crystallization product was naringin with a purity of 83.38%.

关 键 词：蜜柚 黄酮 纯度 重结晶 精制 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]