土壤酚酸胁迫对杨树光合效率参数的影响  

Effects of soil phenol stress on the photosynthetic efficiency parameters of poplar

作　　者：窦志扬 任改 苗德志 邓海瑜 孙宇轩 徐涵 李辉 张光灿[1] DOU Zhiyang;REN Gai;MIAO Dezhi;DENG Haiyu;SUN Yuxuan;XU Han;LI Hui;ZHANG Guangcan(Shandong Agricultural University,Shandong Taishan National Forest Ecosystem National Positioning Research Station,271000,Tai′an,Shandong,China;South China Institute of Environmental Sciences,Ministry of Ecology and Environment,510655,Guangzhou,China;Linyi Yishui County Water Conservancy Engineering Support Center,276000,Linyi,Shandong,China;Linyi University 276000,Linyi,Shandong,China)

机构地区：[1]山东农业大学,山东泰山森林生态系统国家定位研究站,山东泰安271000 [2]生态环境部华南环境科学研究所,广州510655 [3]临沂市沂水县水利工程保障中心,山东临沂276000 [4]临沂大学,山东临沂276000

出　　处：《中国水土保持科学》2022年第5期47-55,共9页Science of Soil and Water Conservation

基　　金：山东省农业科技资金项目子课题“干旱瘠薄山地耐旱保土型优良经济树种高效栽培技术研究与示范(2019LY005)”;国家自然科学基金“杨树响应土壤酚酸胁迫的氮同化机理研究”(31700553);“杨树响应对羟基苯甲酸胁迫中的氧化还原调控机制解析”(31870379)。

摘　　要：为探索土壤酚酸胁迫对杨树光合作用效率的影响特征与生理机制,以盆栽1年生欧美杨107(Populus×euramericana‘Neva’)苗木为试验材料,以杨树第二代林分的土壤酚酸质量浓度(T)为参考值进行盆栽土壤不同酚酸质量浓度处理,分别为0.5T、1.0T、1.5T、2.0T、2.5T、3.0T和0T(对照),测定分析不同酚酸质量浓度土壤下杨树叶片气体交换参数与叶绿素荧光参数的变化。结果表明:1)随着土壤酚酸质量浓度的增大,杨树叶片净光合速率、最大光合速率、表观量子效率以及叶绿素最大光化学效率、实际光化学效率等光合效率参数明显减小。2)低酚酸质量浓度(0.5T~1.5T)时,杨树叶片净光合速率下降的原因以气孔因素限制为主,即光合机构的CO_(2)供应受阻;高酚酸质量浓度(1.5T~3.0T)时,杨树叶片净光合速率下降的原因转变为以非气孔因素限制为主,即光合机构的光合反应活性降低。因此,土壤酚酸胁迫对杨树光合效率具有明显抑制作用,高酚酸质量浓度下光合作用发生明显光抑制;土壤酚酸质量浓度增大时,杨树光合机构可通过增加热耗散实施光保护,是杨树光合作用忍耐土壤酚酸胁迫的一种生理对策。[Background]Studies on phenol stress in poplar have mainly focused on the cumulative effects of soil phenol and inter-root effects,but there is a lack of studies on the effects of phenol in soil on photosynthesis in poplar.The present study aims to reveal the characteristics and mechanisms of soil phenol stress on photosynthetic efficiency of poplar,and to provide a plant physiological basis for a deeper understanding of the inhibitory effect of soil phenol accumulation on the growth of poplar.[Methods]In order to explore the characteristics and physiological mechanism of soil phenol stress on photosynthetic efficiency of poplar,one-year-old potted poplar(Populus×euramericana‘Neva’)seedlings were used as experimental materials,and soil phenol mass concentration(T)of the second generation poplar stand was used as the reference value,(0.5T,1.0T,1.5T,2.0T,2.5T,and 3.0T)and 0T(control)of phenol mass concentration treating soil were set,and the photosynthesis measuring instrument(CIRAS-2)was used to determine the photosynthesis response data of different leaves to 14 photosynthetic active radiations,and the chlorophyll fluorescence parameters were measured using the portable modulation fluorometer(FMS-2).[Results]1)At phenol mass concentration<2.0T,the net photosynthetic rate(P_(n))under the condition of photosynthetically active radiation(PAR)<400μmol/(mol^(2)·s)increased rapidly with PAR,and P_(n) under the condition of PAR>1200μmol/(mol^(2)·s)increased slowly with PAR.The P_(n) under PAR<400μmol/(mol^(2)·s)condition decreased significantly when the phenol mass concentration was>2.0T,and the P_(n) showed a significant decreasing trend with increasing PAR under high light intensity.2)At phenol mass concentrations between 2.5T and 3.0T,the apparent quantum yield(Φ)decreased by 52%-62%compared with the control,the light-saturated net photosynthetic rate(P_(nmax))decreased by 45%-60%compared with the control,the light compensation point(LCP)increased by 12%-13%compared with the control,and the light saturati

关 键 词：酚酸胁迫 光合作用 叶绿素荧光 光化学效率 杨树 

分 类 号：Q945.78[生物学—植物学]