弓形虫免疫作图蛋白1(TgIMP1)表位疫苗的设计及构建  

Design and construction of an epitope vaccine of Toxoplasma gondii immune mapped protein 1(TgIMP1)

作　　者：徐昊志 周贝贝[2] 刘俏俏[3] 董宏杰 代莉莎 谢环环 孙航 王琦 张俊梅 赵桂华[1] 徐超[1] 尹昆[1] XU Hao-zhi;ZHOU Bei-bei;LIU Qiao-qiao;DONG Hong-jie;DAI Li-sha;XIE Huan-huan;SUN Hang;WANG Qi;ZHANG Jun-mei;ZHAO Gui-hua;XU Chao;YIN Kun(Shandong Institute of Parasitic Diseases,Shandong First Medical University&Shandong Academy of Medical Sciences,Jining 272033,Shandong,China;Jining Blood Center;Laizhou City People’s Hospital)

机构地区：[1]山东省寄生虫病防治研究所,山东第一医科大学(山东省医学科学院),山东济宁272033 [2]济宁市中心血站 [3]莱州市人民医院

出　　处：《中国病原生物学杂志》2022年第8期887-891,共5页Journal of Pathogen Biology

基　　金：山东省泰山学者项目工程(No.tsqn202103186);山东省医药卫生科技发展计划项目(No.202101050270);济宁市重点研发计划(2022YXNS152);山东省自然科学基金联合专项(No.ZR2018LH016);山东第一医科大学学术提升计划项目(No.2019QL005);山东省医学科学院医药卫生科技创新工程。

摘　　要：目的设计基于弓形虫免疫作图蛋白1(TgIMP1)抗原蛋白的表位疫苗并进行结构验证。方法分别采用IEDB和ABCpred软件预测TgIMP1蛋白的B细胞抗原表位,分别采用SYFPEITHI和IEDB软件预测TgIMP1蛋白的T细胞抗原表位,运用DNA man比对筛选出T-B联合表位,在PfIMP2同源结构中验证表位性质并设计TgIMP1优势表位盒。结果通过计算机虚拟设计,得到了4条TgIMP1的优势B细胞表位(B1-B4)和2条优势T细胞表位(T1-T2),6条优势表位均处于PfIMP2结构外侧的无规卷曲区,且均为极性表面,适合作为表位疫苗的备选肽段。结论根据预测结果构建了3种TgIMP1优势表位盒,为多价表位疫苗的研发奠定基础。Objective Design an epitope vaccine based on Toxoplasma gondii immune mapped protein 1(TgIMP1)and verified the prediction vaccine using the homologous protein structure.Methods B cell epitopes of TgIMP1 protein were predicted by IEDB and ABCpred software,respectively;T cell epitopes of TgIMP1 protein were predicted by SYFPEITHI and IEDB,respectively.T-B combined epitopes were screened by DNA MAN comparison.The properties of predicted epitopes were verified in PfIMP2 homologous structure.Based on the results,the TgIMP1 dominant epitope boxes were designed and constructed.Results Four dominant B cell epitopes(B1-B4)and two dominant T cell epitopes(T1-T2)of TgIMP1 were successfully predicted by computer virtual design method.All of the predicted dominant epitopes were located on the outside of the PfIMP2 structure,in the random curl zone and with polar surfaces,indicating that they were appropriate as candidates for constructing epitope vaccine.Conclusion Based on the above virtual design results,three TgIMP1 dominant epitope boxes were constructed,laying a foundation for the subsequent development of multivalent epitope vaccines.

关 键 词：免疫作图蛋白1 抗原表位 蛋白三维结构 虚拟设计 弓形虫 

分 类 号：R382.5[医药卫生—医学寄生虫学]