娑罗子中主要活性物质的检测方法  

Detection Method of Main Bioactive Compounds in Aesculi Semen

作　　者：柴莎莎 徐圣[1] Chai Sha-sha;Xu Sheng(School of Biological Engineering,Wuhan Polytechnic,Wuhan 430074,China)

机构地区：[1]武汉职业技术学院生物工程学院,湖北武汉430074

出　　处：《武汉职业技术学院学报》2022年第5期110-114,共5页Journal of Wuhan Polytechnic

基　　金：湖北教育厅百校联百县——高校服务乡村振兴科技支撑行动计划项目“娑罗子中皂苷类物质制备及成分分析”(项目编号:BXLBX1188)。

摘　　要：建立娑罗子中主要活性物质的快速检测方法。娑罗子中的主要活性成分为皂苷类中的七叶皂苷,七叶皂苷的主要存在形式为七叶皂苷A、B、C、D。将样品用乙醚回流提取后,离心取上清定容后过滤备用。采用高效液相色谱分析法测定七叶皂苷A、B、C、D含量,色谱条件:柱温30℃,流速1mL/min,流动相为0.1%磷酸水溶液和乙腈(V/V=36:64),等梯度洗脱,检测波长220 nm。七叶皂苷A、B、C、D在检测范围内有良好的线性,相关系数均在0.9995以上,检测限在0.05~0.2μg/mL之间,定量限在0.2~0.8μg/mL之间,加标回收率在86.27%~97.21%之间。此方法适用于天然产物娑罗子中七叶皂苷的含量测定。A rapid detection method for the main bioactive compounds in Aesculi Semen is established.The main bioactive compounds in Aesculi Semen were the aescins of saponins.The basic form of beings were aescin A,aescin B、aescin C and aescin D.After being refluxed and extracted with ether,the supernatant was centrifuged to obtain a constant volume,and then filtered for later use.To establish an HPLC method for the simultaneous content determination of aescin A,aescin B、aescin C and aescin D.HPLC method:Column temperature 30℃,gradient elution manne.The analysis of methanol extract of the aescins was performed on 30℃of Column temperature,the mobile phase comprising of 0.1%phosphoric acid-water and acetonitrile flowing at 1.0 mL/min in a isocratic elution manner,and the detection wavelength was set at 220 nm.The 4 aescins showed good linearity in the range of determination,the correlation coefficients were all above 0.9995.The limits of detection were 0.05-0.2μg/mL,and the limits of quantification were 0.2-0.8μg/mL.The recoveries of samples at 3 levels were 85.12%~97.21%.This method is simple,rapid and accurate,which is suitable for the detection of aescins in Aesculi Semen.

关 键 词：娑罗子 高效液相 快速检测 

分 类 号：R284.1[医药卫生—中药学]