以聚中性红为介体的生化需氧量微生物传感器的研制  

Preparation of electrochemical biochemical oxygen demand biosensor mediated by polyneutral red

作　　者：周密 颜家保 胡杰 鲍彦舟 孙玲玉 ZHOU Mi;YAN Jia-bao;HU Jie;BAO Yan-zhou;SUN Ling-yu(Hubei Province Key Laboratory of Coal Conversion and New Carbon Materials,School of Chemistry and Chemical Engineering,Wuhan University of Science and Technology,Wuhan 430081,China)

机构地区：[1]武汉科技大学化学与化工学院,煤转化与新型炭材料湖北省重点实验室,湖北武汉430081

出　　处：《现代化工》2022年第10期247-251,256,共6页Modern Chemical Industry

基　　金：湖北省科技创新专项重大项目(2017ACA179)。

摘　　要：制备了一种用于生化需氧量检测的新型微生物传感器,选用玻碳电极为基底电极、海藻酸钠(SA)改性的聚乙烯醇(PVA)凝胶为微生物包埋载体、地衣芽孢杆菌为固定化微生物,将氧化还原介体中性红(NR)电聚合到微生物细胞表面形成聚中性红(PNR),并对传感器电化学性能进行了探究。结果表明,与微生物敏感膜内细胞结合的PNR能有效保持氧化还原性能,从而促进微生物与电极表面的电子转移。该电极在4~24 mg/L BOD标准溶液中呈现良好的线性响应性能,检出限2 mg/L(S/N=3),响应时间400 s。固定化微生物存活率80%以上,最适酸碱环境为中性,最适温度为35℃。微生物传感电极在2个月内保持着良好的稳定性。A novel electrochemical microbial sensor is prepared for the detection of biochemical oxygen demand.Taking glassy carbon electrode as the substrate electrode,sodium alginate(SA)modified polyvinyl alcohol(PVA)gel as a microbial carrier,bacillus licheniformis as the immobilized microorganism,and neutral red as a redox mediator is polymerized onto the surface of microbial cells to form polyneutral red(PNR).The electrochemical performance of the sensor is studied.Results show that PNR combined with cells in microbial sensitive membrane can effectively maintain redox performance,which can promote electron transfer between microorganisms and electrode surface.The electrode shows good linear response in 4-24 mg·L^(-1) BOD standard solution,with a limit detection of 3 mg·L^(-1)(S/N=3)and a response time of 400 s.The survival rate of immobilized microorganisms exceeds 80%.Neutral environment is the optimum for the immobilized microorganisms and the optimum temperature for the immobilized microorganisms is 35℃.This microbial sensing electrode has maintained a good stability in two months.

关 键 词：生化需氧量 微生物传感器 玻碳电极 氧化还原介体 聚中性红 

分 类 号：X832[环境科学与工程—环境工程]