基于超高效液相色谱-串联质谱技术快速测定果梅中化学成分  

Rapid determination of chemical components in fruit plum based on ultra performance liquid chromatography-tandem mass spectrometry technique

作　　者：夏梦秋 王星晨 杨兰香 岳婧怡 王瑞 曹侃[1,2] 程旺开 XIA Mengqiu;WANG Xingchen;YANG Lanxiang;YUE Jingyi;WANG Rui;CAO Kan;CHENG Wangkai(Wuhu Institute of Technology,Wuhu 241000,China;Wuhu Life and Health Engineering and Technology Research Center,Wuhu 241000,China)

机构地区：[1]芜湖职业技术学院,安徽芜湖241000 [2]芜湖生命健康工程技术研究中心,安徽芜湖241000

出　　处：《食品与发酵工业》2022年第20期260-264,271,共6页Food and Fermentation Industries

基　　金：2022年度校级科学研究项目(wzyzr202231);2019年安徽省教育厅质量工程项目(皖教秘高[2019]94号,2019cxtd023)。

摘　　要：采用超高效液相色谱-串联质谱技术对果梅的化学成分进行快速测定。采用ZORBAX SB-C18(3.0 mm×100 mm,3.5μm)色谱柱,流动相为水(含体积分数0.2%甲酸)(A)和乙腈(含体积分数0.2%甲酸)(B),采用梯度洗脱方式,柱温30℃,流速为0.2 m/min,进样量为1μL。从果梅中成功测定了16种化学成分,主要包括10种有机酸、4种黄酮类、5-羟甲基糠醛和苦杏仁苷。该文成功快速测定了果梅中主要的化学成分,为进一步研究果梅的药效基础和作用机制提供理论基础。ultra performance liquid chromatography-tandem mass spectrometry was used to determine the chemical components in fruit plum.The analysis conditions were as follows.The chromatographic separation was performed using a ZORBAX SB-C18(3.0 mm×100 mm,3.5μm)column,and the temperature of the column was maintained at 35℃.The mobile phases were 0.2%formic acid(A)and acetonitrile(including 0.2%formic acid)(B),respectively,using a binary gradient elution at the flow rate of 0.2 mL/min.Results showed that sixteen chemical components were successfully determined in fruit plum,including 10 organic acids,4 flavonoids,5-hydroxymethylfurfural,and bitter amygdidine.The main chemical compositions of fruit plum were successfully and quickly determined in this paper and the results could provide the theoretical basis for further study of the efficacy basis and mechanism of action.

关 键 词：果梅 超高效液相色谱-串联质谱法 化学成分 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]