3株枯草芽孢杆菌对烟草白粉病的防效  

Control Effect of Three Bacillus subtilis Strains on Tobacco Powdery Mildew

作　　者：蔡永占 王瑞宝[1] 白涛[1] 刘有才[1] 韩小女[2] 王斌 华小兵 李诚 毛明林 字文霖 无 CAI Yongzhan;WANG Ruibao;BAI Tao;LIU Youcai;HAN Xiaonv;WANG Bin;HUA Xiaobing;LI Cheng;MAO Minglin;ZI Wenlin;无(Qujing Branch of Yunnan Provincial Tobacco Company,Qujing,Yunnan 655000;Xuanwei Agricultural Technology Extension Center,Xuanwei,Yunnan 655400;National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains,Kunming 650217;Yunnan Microbial Fermentation Engineering Research Center Co.,Ltd.,Kunming 650217)

机构地区：[1]云南省烟草公司曲靖市公司,云南曲靖655000 [2]宣威市农业技术推广中心,云南宣威655400 [3]微生物菌种筛选与应用国家地方联合工程研究中心,昆明650217 [4]云南省微生物发酵工程研究中心有限公司,昆明650217

出　　处：《中国农学通报》2022年第25期125-129,共5页Chinese Agricultural Science Bulletin

基　　金：云南省烟草公司科技计划重点项目“曲靖烟草中后期白粉病、赤星病生物防控技术研究与示范”(2019530000241008)。

摘　　要：为探明3株枯草芽孢杆菌对‘云烟87’白粉病的防效,以枯草芽孢杆菌制得的悬浮剂、菌悬液和低毒化学农药为试验材料,开展其对白粉病防效的研究。室内结果表明,YWF-19-0068、YWF-19-0070、YWF-19-0071生防制剂10倍液对烟草白粉病的防效分别达70.49%、61.15%、69.71%,50倍菌悬液对白粉孢子的萌发抑制率分别达56.92%、56.92%、58.46%。室外结果表明,3株枯草芽孢杆菌制剂不同剂量用药10天后,50倍液的大田防效最优,分别可达46.85%、65.50%、51.70%;选取YWF-19-0070菌株悬浮制剂分别在甲基硫菌灵全量和半量用药一次后,每隔3天使用一次,最后一次用药10天后,甲基硫菌灵全量处理的防效达77.73%以上;甲基硫菌灵半量处理的防效达60.23%以上。To clarify the control effects of three Bacillus subtilis strains on powdery mildew of flue-cured tobacco variety‘Yunyan 87’,suspensions,bacterial suspension,and suspension agent(SC)prepared by B.subtilis and low-toxic chemical pesticides were used as test materials to carry out research on their control effects on powdery mildew.The indoor results showed that:YWF-19-0068,YWF-19-0070 and YWF-19-0071 biocontrol agents of 10 times dilution had the control effect of 70.49%,61.15%and 69.71%,respectively.And 50 times dilution of bacterial suspension agents had the germination inhibition rate on the powdery mildew spore of 56.92%,56.92%and 58.46%,respectively.The outdoor results showed that:10 days after the application of the three biocontrol agents at different dosages,the control effects of 50 times dilution of bacterial suspension agents were the best of 46.85%,65.50%,and 51.70%,respectively.After full and half doses of thiophanate were used once,YWF-19-0070(SC)was applied once every 3 days.10 days after the last application,the control effect of the full dosage thiophanate methyl treatment was more than 77.73%,and the control effect of the half dosage thiophanate methyl treatment was more than 60.23%.

关 键 词：枯草芽孢杆菌 悬浮制剂 烟草 白粉病 防效 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]