外源亚精胺处理方式对盐胁迫下草莓生理生化的影响  

Effects of exogenous spermidine treatment on physiological and biochemical characteristics of strawberry under salt stress

作　　者：石硕 宋思豪 遆月迎 杨爱珍[1] 郭家选[1] 高凡[1] SHI Shuo;SONG Sihao;TI Yueying;YANG Aizhen;GUO Jiaxuan;GAO Fan(Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs/Department of Resources and Environment,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]农业农村部华北都市农业重点实验室/北京农学院资源与环境系,北京102206

出　　处：《北京农学院学报》2022年第4期32-36,共5页Journal of Beijing University of Agriculture

基　　金：国家自然科学基金项目(32072516,32030100);北京市自然科学基金-市教委联合资助项目(KZ202010020028)。

摘　　要：【目的】探究设施草莓抗盐的最适外源亚精胺(spermidine,Spd)施用方式。【方法】采用100 mM NaCl溶液模拟盐胁迫,叶面喷施和根部添加、预先处理和胁迫后处理的0.1 mM外源Spd处理方式,测定草莓叶片的净光合速率(P_(n))、蒸腾速率(T_(r))、气孔导度(G_(s))、胞间CO_(2)浓度(C_(i))、PSⅡ最大光化学效率(F_(v)/F_(m))、相对电导率(EC)、丙二醛(MDA)和脯氨酸(Pro)含量等指标。【结果】盐胁迫降低了草莓叶片F_(v)/F_(m)、P_(n)、G_(s)和T_(r),升高了叶片的EC及根叶的MDA水平;其中在盐胁迫的第5天,预先叶面喷施处理叶片的P_(n)、G_(s)、T_(r)和F_(v)/F_(m)值最高,分别为单独盐胁迫处理的133%、211%、192%和108%,EC值和MDA含量分别仅为单独盐胁迫的89.10%和71.69%,且低于其他Spd处理方式。叶面喷施和根部添加Spd处理均可诱导草莓叶片的Pro含量升高,缓解盐胁迫对草莓叶片的光合抑制及氧化损伤,但预先Spd处理的叶片及根系中Pro含量高于后Spd处理。【结论】外源Spd叶面或根部施用均有助于提高草莓的抗盐性,其中预先外源叶面喷施Spd对提高草莓抗盐性的效果最好。[Objective]The aim of the present study is to determine if different treatments of exogenous spermidine(Spd)affected the physiological and biochemical characteristics of strawberry under salt stress.[Methods]Benihoppe was used as experimental material,and 100 mM NaCl solution was used to simulate salt stress.The experimental design included six treatments(T):(a)CK;0 mM NaCl+0 mM Spd,(b)T1;100 mM NaCl+0 mM Spd,(c)T2;100 mM NaCl stress after leaf add 0.1 mM Spd for 24 h,(d)T3;Leaf add 0.1 mM Spd after 100 mM NaCl stress for 24h,(e)T4;100 mM NaCl stress after root add 0.1 mM Spd for 24 h,(f)T5;Root add 0.1 mM Spd after 100 mM NaCl stress for 24h.The optimal/maximal quantum yield of PSⅡ(F_(v)/F_(m)),net photosynthetic rate(P_(n)),transpiration rate(T_(r)),stomatal conductance(G n),intercellular CO_(2)concentration(C_(i)),relative conductivity(EC),malondialdehyde(MDA)and proline(Pro)content were analyzed in this study.[Results]The results showed that the levels of Pn,Gs,Tr and F_(v)/F_(m)in leaves were significantly decreased,but EC value of leaves and MDA content of roots and leaves were dramatically increased,when strawberries were subjected to salt stress.On the fifth day of salt stress on strawberries,the levels of P_(n),G_(s),T_(r)and F_(v)/F_(m)in leaves with pre-foliar spraying of exogenous Spd were the highest,which were 133%,211%,192%and 108%of those under salt stress alone,respectively;however,the EC value and MDA content in leaves by Spd pretreatment were only 89.10%and 71.69%of those under salt stress alone,respectively.Both foliar spraying and root addition of exogenous Spd could induce a significant increase of Pro content in strawberry leaves and alleviate the photosynthetic inhibition and oxidative damage of strawberry leaves caused by salt stress,but the levels of Pro in leaves and roots by Spd pretreatment were higher than those by Spd post-treatment.[Conclusion]The application of exogenous Spd on the leaves or roots can both enhance salt tolerance of strawberries,and the best application method is

关 键 词：草莓 外源亚精胺 盐胁迫 气体交换参数 脯氨酸 

分 类 号：Q945.78[生物学—植物学]