姜黄素对类风湿关节炎成纤维样滑膜细胞增殖、凋亡、侵袭与迁移的影响  

Effects of curcumin on proliferation, apoptosis, invasion and migration of fibroblast synovial cells in rheumatoid arthritis

作　　者：蔡振富 朱程章 于其华 CAI Zhen-fu;ZHU Cheng-zhang;YU Qi-hu(Graduate School of Anhui University of Traditional Chinese Medicine,Fuyang 230012;Department of Orthopaedics,Wuhu Hospital of Traditional Chinese Medicine,Wuhu 241003;Department of Orthopaedics and Traumatology,Taihe County Hospital of Traditional Chinese Medicine,Anhui University of Chinese Medicine,Fuyang 236699,China)

机构地区：[1]安徽中医药大学研究生院,安徽阜阳230012 [2]芜湖市中医医院骨科,安徽芜湖241003 [3]安徽中医药大学附属太和县中医院骨伤科,安徽阜阳236699

出　　处：《解剖科学进展》2022年第3期331-334,共4页Progress of Anatomical Sciences

基　　金：安徽高校省级自然科学研究项目(KJ2018A352)。

摘　　要：目的 研究姜黄素对TNF-α诱导的HFLS-RA细胞增殖、凋亡、侵袭、迁移的影响及其与Wnt/β-catenin蛋白的关系。方法HFLS-RA细胞分为对照组、诱导组、姜黄素5μmol/L组和姜黄素10μmol/L组。MTT实验检测各组细胞增殖能力;流式细胞术检测各组细胞凋亡情况;Transwell实验和划痕实验检测细胞侵袭迁移能力;Western blot检测细胞中Wnt/β-catenin及TNF-α蛋白表达水平。结果 与对照组相比,诱导组和姜黄素组细胞增殖、侵袭与转移能力显著增强,细胞凋亡数减少,Wnt、β-catenin和TNF-α蛋白表达明显增加;与诱导组相比,姜黄素组细胞增殖、侵袭与转移能力显著降低,细胞凋亡数显著增加,Wnt、β-catenin和TNF-α蛋白表达显著下降。结论 姜黄素抑制TNF-α诱导的HFLS-RA细胞增殖、侵袭与迁移,并诱导其凋亡,其机制可能与调控Wnt/β-catenin蛋白有关。Objective To study the effects of curcumin on proliferation, apoptosis, invasion and migration of TNF-α-induced HFLS-RA cells and its relationship with Wnt/β-catenin protein. Methods HFLS-RA cells were cultured and divided into control group, induction group, curcumin 5μmol/L group and curcumin 10μmol/L group. Cell proliferation was measured by MTT assay. Cell apoptosis was detected by flow cytometry. Transwell assay and scratch assay were used to detect the invasion and migration of cells. The expression of Wnt/β-catenin and TNF-α were detected by Western blot. Results Compared with the control group, the proliferation, invasion and migraition of cells in the induced group and curcumin group were significantly enhanced, the number of apoptosis cells was decreased, and the expressions of Wnt, β-catenin and TNF-α were significantly increased. Compared with the induction group, the proliferation, invasion and metastasis of cells in curcumin group were significantly decreased, the number of apoptosis cells was significantly increased, and the expression of Wnt, β-catenin and TNF-α were significantly decreased. Conclusion Curcumin inhibits the proliferation, invasion and migration of HFLS-RA cells induced by TNF-α, and induces apoptosis, which may be related to the regulation of Wnt/β-catenin protein.

关 键 词：姜黄素 HFLS-RA 凋亡 侵袭 转移 WNT/Β-CATENIN 

分 类 号：R593.22[医药卫生—内科学]