菟丝子中一个新的吲哚类生物碱  

A new indole alkaloid from Cuscutae Semen

作　　者：杜锟 薛贵民 支燕乐 赵珍珠 司盈盈 陈辉[1] 马金莲 DU Kun;XUE Gui-min;ZHI Yan-le;ZHO Zhen-zhu;SI Ying-ying;CHEN Hui;MA Jin-lian(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China;Academy of Chinese Medical Sciences,Henan University of Chinese Medicine,Zhengzhou,Henan 450046,China)

机构地区：[1]河南中医药大学药学院,郑州450046 [2]河南中医药大学第一附属医院,郑州450000 [3]河南中医药大学中医药科学院,郑州450046

出　　处：《天然产物研究与开发》2022年第10期1713-1717,共5页Natural Product Research and Development

基　　金：河南省科技攻关(182102310288,212102310314);河南中医药大学博士科研基金(BSJJ2018-19,00104311-2019-7)。

摘　　要：研究中药菟丝子(Cuscutae Semen)的化学成分。采用Sephadex LH-20和硅胶等柱色谱以及反相制备型HPLC等方法分离与纯化,运用NMR、MS等波谱技术鉴定化合物结构。从菟丝子的甲醇提取物中分离得到6个化合物,分别鉴定为菟丝子新碱A(1)、金丝桃苷(2)、槲皮素-3-O-β-D-半乳糖-(2→1)-β-D-芹糖苷(3)、槲皮素(4)、山奈酚(5)、芝麻素(6)。其中化合物1为一个新的β-卡波林类生物碱。化合物2和5对组蛋白赖氨酸特异性去甲基化酶1(lysine specific demethylase 1,LSD1)显示出较强的抑制作用,IC_(50)分别为5.74±0.49和3.94±0.27μmol/L。To study the chemical constituents from Cuscutae Semen.The compounds were isolated and purified by various modern chromatographic techniques,including column chromatography(Sephadex LH-20 or silica gel)and preparative HPLC,and their structures were identified by spectroscopic data and physicochemical properties.Six compounds were isolated from the MeOH extract of Cuscutae Semen,which were elucidated as cuscutalin A(1),hyperoside(2),quercetin 3-O-β-D-apiofuranosyl-(1→2)-β-D-galactoside(3),quercetin(4),kaempferol(5),and sesamin(6).Compound 1 is a newβ-carboline alkaloid.Compounds 2 and 5 exhibited strong inhibitory activity against lysine specific demethylase 1 with the IC_(50) values of 5.74±0.49 and 3.94±0.27μmol/L,respectively.

关 键 词：菟丝子 化学成分 吲哚类生物碱 黄酮苷 LSD1抑制活性 

分 类 号：R284.1[医药卫生—中药学]