花生抗锈病种质筛选及抗性QTL分析  

Novel genotypes and quantitative trait locus for rust resistance in peanut

作　　者：成良强 郭建斌[2] 李威涛 黄莉[2] 罗怀勇[2] 刘念[2] 周小静[2] 陈伟刚[2] 王军[1] 吕建伟 胡廷会 饶庆琳 雷永[2] 廖伯寿[2] 姜慧芳 CHENG Liang-qiang;GUO Jian-bin;LI Wei-tao;HUANG Li;LUO Huai-yong;LIU Nian;ZHOU Xiao-jing;CHEN Wei-gang;WANG Jun;LYU Jian-wei;HU Ting-hui;RAO Qing-lin;LEI Yong;LIAO Bo-shou;JIANG Hui-fang(Academy of Agricultural Sciences of Guizhou Province/Oil Research Institute of Gui Zhou,Guiyang 550006,China;Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China)

机构地区：[1]贵州省农业科学院/贵州省油料研究所,贵州贵阳550006 [2]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2022年第5期1074-1080,共7页Chinese Journal of Oil Crop Sciences

基　　金：贵州省基础研究计划(自然科学)(黔科合基础-ZK[2022]一般290);贵州省农科院青年基金(黔农科院青年基金[2018]11号);农业部油料作物生物学与遗传育种重点实验室开放课题(KF2020008);贵州省科技计划基础研究(科学技术基金)(黔科合基础-ZK[2021]一般134);财政部和农业农村部:国家现代农业产业技术体系资助。

摘　　要：锈病是影响花生产量和品质的主要真菌性病害。本研究以“中花10号×ICG 12625”构建的重组自交系群体(RIL)为材料,在武昌和阳逻两个环境中对其F_(8)群体进行锈病抗性鉴定,获得了8份抗锈病材料(QT0348、QT0368、QT0400、QT0402、QT0419、QT0458、QT0463和QT0485)。利用前期构建的遗传图谱进行QTL分析,检测到10个与锈病抗性相关的QTL,贡献率为4.54%~10.78%,分布于7条染色体上。其中,qBB06.1和qBB06.4为重复一致的QTL,贡献率为10.76%。根据侧翼标记在物理图谱进行比对,发现对应区段含有193个基因,其中178个基因被注释,功能注释基因中有1个NBS-R基因。本研究结果为花生抗锈病的遗传改良奠定了基础。Rust is a major fungal disease affecting peanut yield and quality.In this study,a recombinant inbred line population(RIL)constructed from“Zhonghua 10×ICG 12625”was used as material.The Fgeneration population was tested for rust resistance in two environments,Wuchang and Yangluo.8 rust-resistant lines(QT0348,QT0368,QT0400,QT0402,QT0419,QT0458,QT0463 and QT0485)were obtained.QTL analysis using the pre-constructed genetic map detected 10 QTLs associated with rust resistance,with 4.54%-10.78% phenotypic variation explained(PVE),distributed on 7 chromosomes.Among them,q BB06.1 and q BB06.4 were repeat consistent QTLs with contribution rates of 10.76%.The corresponding segment in the physical map contained 193 genes according to the flanking markers,of which 178 genes were annotated,and one NBS-R gene was among the functionally annotated genes.The results of this study provide a basis for genetic improvement of rust resistance in peanut.

关 键 词：花生 锈病 重组自交系 抗锈病新种质 QTL 

分 类 号：S565.2[农业科学—作物学]