亚甲蓝与β-胡萝卜素双褪色法在大豆种子脂氧酶缺失育种上的应用研究  

Methylene blue and β-carotene double fading method in soybean breeding of lipoxygenase free

作　　者：王先虚 范慧明 藕冉 王磊[2,3] 王遂 姜妍 王绍东[1,4] WANG Xian-xu;FAN Hui-ming;OU Ran;WANG Lei;WANG Sui;JIANG Yan;WANG Shao-dong(Key Laboratory of Soybean Biology of Education Ministry,Northeast Agricultural University,Harbin 150030,China;MOA Key Laboratory of Biology and Genetic Improvement of Soybean,Nanjing Agricultural University,Nanjing 210095,China;Mudanjiang Soybean Research Center,Mudanjiang 157041,China;National Research Center of Soybean Engineering and Technology,Harbin 150028,China)

机构地区：[1]东北农业大学大豆生物学教育部重点实验室,黑龙江哈尔滨150030 [2]南京农业大学农业部大豆生物学与遗传育种重点实验室,江苏南京210095 [3]牡丹江大豆研发中心,黑龙江牡丹江157041 [4]东北农业大学国家大豆工程技术研究中心,黑龙江哈尔滨150028

出　　处：《中国油料作物学报》2022年第5期1123-1129,共7页Chinese Journal of Oil Crop Sciences

基　　金：科技部十三五重点研发计划(2017YFD0101300);黑龙江省“百千万”工程科技重大专项(SC2019ZX16B0039)。

摘　　要：F大豆种子脂氧酶(Lipoxygenase,Lox-1,2,3)三个同工酶缺失性状筛选鉴定方法的开发,对于脂氧酶缺失的无腥味大豆育种及其表型鉴定具有重要意义。本研究利用脂氧酶Lox-1,2,3三个同工酶在不同pH下对亚甲蓝和β-胡萝卜素褪色反应的颜色变化,通过痕量取样,精准鉴定F_(2)分离世代出现的Lox-1,2,3野生型、Lox-1,2,3缺失型、Lox-1,2缺失型、Lox-3缺失型四种表现型,该检测手段不影响含目标性状的个体的正常继代扩繁,为脂氧酶完全缺失大豆新品种的培育及其表型鉴定提供技术支持。The development of screening and identification methods for three isozyme lacking characteristics of lipoxygenase(lox-1,2,3)is significance to the breeding and phenotypic identification of odorless soybean with lipoxygenases lacking in Fsoybean seeds.In this study,methylene blue and β-carotene fading reactions at different pH values of three isoenzymes Lox-1,2,3 were used to accurately identify four phenotypes of Lox-1,2,3 wild type,Lox-1,2,3 free,Lox-1,2 free,and Lox-3 free in F_(2)generation by trace sampling.The identification had no impacts on breeding of the selected individuals with target traits and could provide technical support for breeding and phenotypic identification of new soybean varieties with null lipoxygenases deficiency.

关 键 词：F2大豆种子 脂氧酶缺失育种 痕量取样 快速检测 亚甲蓝和β-胡萝卜素褪色反应 

分 类 号：S565.103[农业科学—作物学]