基于邻苯二胺与Ag^(+)构建荧光探针检测邻苯二酚  

A fluorescent probe based on o-phenylenediamine and silver ions for the detection of catechol

作　　者：于梦娣 葛红莲 叶存玲[1] 王治科 范顺利 YU Meng-di;Ge Hong-lian;YE Cun-ling;WANG Zhi-ke;FAN Shun-li(College of Chemistry and Chemical Engineering,Henan Normal University,Xinxiang 453007,China;School of Environment,Henan Normal University,Xinxiang 453007,China)

机构地区：[1]河南师范大学化学化工学院,河南新乡453007 [2]河南师范大学环境学院,河南新乡453007

出　　处：《化学研究与应用》2022年第10期2297-2303,共7页Chemical Research and Application

基　　金：河南省高等学校重点科研项目(22B610003)资助;河南省科技攻关项目(222102320354)资助。

摘　　要：本文利用Ag^(+)氧化邻苯二胺(OPD),生成银纳米颗粒(AgNPs)和橘黄色的荧光物质2,3-二氨基吩嗪(OPDox)。邻苯二酚(CC)有2个相邻的酚羟基,在弱碱性介质中,容易通过氧化自聚黏附在AgNPs表面,抑制了AgNPs对Ag^(+)与OPD之间反应的催化作用,导致生成OPDox的量减少,荧光强度下降。据此,建立一种原位构建OPDox荧光探针测定水中CC的方法。在最佳实验条件下,体系荧光强度的变化(ΔF)与CC浓度在0.080~1.0μM范围内呈现良好的线性关系,该方法的检出限为0.024μM。一些常见的酚类化合物,如间苯二酚(RC)、对苯二酚(HQ)等不干扰测定。该方法灵敏,操作简单,可以应用于水样中邻苯二酚的选择性检测。In this paper,o-phenylenediamine(OPD)was oxidized by Ag^(+)to form silver nanoparticles(AgNPs)and 2,3-diaminophenazine(OPDox)which emitted a yellow fluorescence.Catechol(CC)has two adjacent phenolic hydroxyl groups.In weakly alkaline conditions,CC easily deposited onto the surface of AgNPs by its oxidation and self-polymerization.This inhibited the catalytic effect of AgNPs on the reaction of Ag^(+)and OPD,resulting in a decrease in the amount of generated OPDox,and correspondingly the fluorescence intensity of Ag^(+)-OPD system decreased.Therefore,a fluorescent method for the detection of CC was proposed based on the fabrication of in situ OPDox.Under the optimal conditions,the concentration of CC in the range of 0.080~1.0μM exhibited a good linear relation with the change of fluorescence intensity.The limit of detection of the proposed method was calculated to be 0.024μM.Some phenolic compounds such as resorcinol(RC),hydroquinone(HQ)and etc.had almost no effects on the detection of CC.The proposed method is sensitive,simple,and possesses great potential in the selective detection of CC in water samples.

关 键 词：邻苯二胺 原位荧光探针 邻苯二酚 

分 类 号：O657.3[理学—分析化学]