呼吸道病原体抗体检测的干扰现象及其干扰因素回顾性研究  

Retrospective study on interference phenomenon and interference factors of low-risk pathogen antibody detection

作　　者：王国镇[1] 张洋[2] 刘辉[1] 石玉惠[1] 韩呈武[1] 曹永彤[1] 张铁[1] Wang Guozhen;Zhang Yang;Liu Hui;Shi Yuhui;Han Chengwu;Cao Yongtong;Zhang Tie(Department of Clinical Laboratory,China-Japan Friendship Hospital,Beijing 100029,China;Department of Infectious Disease,China-Japan Friendship Hospital,Beijing 100029,China)

机构地区：[1]中日友好医院检验科,北京100029 [2]中日友好医院感染疾病科,北京100029

出　　处：《中国医刊》2022年第11期1215-1220,共6页Chinese Journal of Medicine

基　　金：北京市朝阳区科技计划课题(CYSF-1835);北京市临床重点专科项目(2020年度)。

摘　　要：目的分析院内呼吸道病原体抗体阳性样本的来源和阳性分布情况,并分析其干扰因素。方法回顾性分析2017年1月至2019年9月在中日友好医院进行呼吸道病原体检测的15个主要科室就诊患者EB(Epstein-Barr)病毒衣壳抗原(EB VCA)IgM、IgA、IgG抗体与巨细胞病毒(cytomegalovirus,CMV)IgM、IgG抗体的阳性率。根据抗核抗体和类风湿因子检测结果进行分组,抗核抗体或类风湿因子任一阳性为阳性组,抗核抗体和类风湿因子均为阳性为双阳性组,抗核抗体和类风湿因子均为阴性为双阴性组。进一步分析类风湿性关节炎、系统性红斑狼疮、肌炎、干燥综合征的呼吸道病原体抗体阳性率及其与自身抗体等指标的相关性。结果全院15个主要科室患者的CMV IgM、CMV IgG阳性率不同,差异有显著性(P<0.001)。其中肿瘤科患者CMV IgM阳性率最高(15.79%),风湿免疫科次之(12.20%)。风湿免疫科与消化科、血液科、外科等9个科室的CMV IgG阳性率比较,差异有显著性(P<0.05)。15个主要科室的EB VCA IgM、EB VCA IgG、EB VCA IgA阳性率不同,差异有显著性(P<0.001)。风湿免疫科与儿科、消化科以及感染科的EB VCA IgG阳性率比较,差异有显著性(P<0.05)。风湿免疫科中类风湿关节炎、系统性红斑狼疮、肌炎、干燥综合征及其他疾病患者的CMV IgM、CMV IgG、EB VCA IgA阳性率不同,差异有显著性(P<0.05)。抗核抗体阳性患者CMV IgM、EB VCA IgA的阳性率明显高于抗核抗体阴性者(P<0.001),且抗核抗体阳性率与CMV IgM、EB VCA IgA的阳性率呈正相关(P<0.001)。类风湿因子阳性者的EB VCA IgM、EB VCA IgA阳性率高于阴性者,EB VCA IgG阳性率低于阴性者,差异均有显著性(P<0.05)。抗核抗体和类风湿因子双阳者CMV IgM、EB VCA IgA阳性率高于单阳性者(P<0.05)。结论全院15个主要科室CMV IgM、CMV IgG阳性率差异有显著性,而风湿免疫科患者相关病原体的阳性率均高于其他科室患者,�Objective To analyze the source and distribution of positive samples of low-risk pathogen antibodies in China-Japan Friendship Hospital,evaluate the correlation between samples and clinical manifestations,and analyze whether there are interference factors affecting the test results.Method We performed retrospective statistical analysis of the positive rates of antibodies such as IgM,IgA,and IgG provoked by Epstein-Barr virus capsid antigen(EB VCA)and that of antibodies such as IgM and IgG provoked by cytomegalovirus(CMV).Based on the test results,we classified antinuclear antibody and rheumatoid factor into three groups:single positive group in which either antinuclear antibody or rheumatoid factor is positive,double positive group in which both antinuclear antibody and rheumatoid factor are positive,and double negative group in which both antinuclear antibody and rheumatoid factor are negative.Furthermore,we analyzed the positive rate of the antibodies of respiratory tract pathogen of patients with diseases such as rheumatoid arthritis,systematic lupus erythematosus,myositis and sjogren’s syndrome from department of rheumatology and immunology,and the correlation of these diseases with indexes of autoantibodies and others was analyzed.Result The positive rates of CMV IgM and CMV IgG of 15 departments of the hospital were different,and the differences were statistically significant(P<0.001).Among them,the positive rate of CMV IgM was the highest in department of oncology(15.79%),followed by department of rheumatology and immunology(12.20%).There was a statistically significant difference in the positive rate of CMV IgG between department of rheumatology and immunology and other departments in the hospital(P<0.05).The positive rates of EB VCA IgM,EB VCA IgG,and EB VCA IgA were different among 15 departments,and the differences were statistically significant(P<0.001).There was a statistically significant difference in EB VCA IgG positive rates among samples from the department of rheumatology and immunology,depar

关 键 词：巨细胞病毒 EB病毒 抗核抗体 类风湿因子 

分 类 号：R446[医药卫生—诊断学]