绝经期H型高血压大鼠模型的建立  

Establishment of rat model of menopausal H-type hypertension

作　　者：谢容 陈建 陈键 洪秀琴 XIE Rong;CHEN Jian;CEHN Jian;HONG Xiuqing(Research Management Division,the First Affiliated Hospital of Hunan Normal University/Hunan Provincial People’s Hospital,Changsha,Hunan 410005,China;Key Laboratory for Molecular Epidemiology,Changsha,Hunan 410013,China;Xiangtan Center for Disease Control and Prevention,Xiangtan,Hunan 411199,China)

机构地区：[1]湖南师范大学附属第一医院/湖南省人民医院科研部,长沙410005 [2]湖南省分子流行病学重点实验室,长沙410013 [3]湖南省湘潭市疾病预防控制中心,411199

出　　处：《重庆医学》2022年第20期3434-3437,3444,共5页Chongqing medicine

基　　金：国家自然科学基金项目(81773530);湖南省自然科学基金项目(2020JJ4047);湖南省长沙市科技计划项目(kq1801096)。

摘　　要：目的探索绝经期H型高血压大鼠模型的构建方法。方法选取6~8周龄雌性自发性高血压大鼠27只分为con组(卵巢切除假手术+普通饮水)、2%met组(卵巢切除假手术+2%蛋氨酸饮水)和ovx+2%met组(双侧卵巢切除+2%蛋氨酸饮水),每组9只。观察每组大鼠的周饮水量和收缩压(SBP)变化,检测血清同型半胱氨酸(Hcy)、雌二醇(E2)和其他血液生化指标水平。结果2%met组和ovx+2%met组大鼠周饮水量均明显低于con组(P<0.01),而2%met组和ovx+2%met组大鼠周饮水量无明显差异(P>0.05)。喂养第10周,2%met组和ovx+2%met组大鼠血清Hcy水平均明显高于con组(P<0.01),喂养第12周,2%蛋氨酸饮水喂养的大鼠血清Hcy>10μmol/L,H型高血压大鼠模型建立成功。喂养第2周,ovx+2%met组大鼠血清E2水平明显低于con组和2%met组(P<0.01)。2%met组和ovx+2%met组大鼠SBP在各时间点均高于con组,且从第8周期开始差异有统计学意义(0.05或P<0.01),而2%met组与ovx+2%met组大鼠SBP在各时间点均无明显差异(P>0.05)。结论SHR大鼠去卵巢手术结合饮用2%蛋氨酸水12周可以构建绝经期H型高血压大鼠模型。Objective To explore the method of the establishment of menopausal H-type hypertensive rat model.Methods Twenty-seven female spontaneous hypertensive rats(SHR)aged 6-8 weeks old were selected and divided into the con group[ovariectomy(OVX)sham operation+ordinary drinking water],2%met group[OVX sham operation+2%methionine(Met)drinking water]and OVX+2%met group(bilateral ovx+2%Met drinking water),9 rats each group.The changes of weekly drinking water intake and systolic blood pressure(SBP)in each group were observed.The serum levels of homocysteine(Hcy),estradiol(E2)and other blood biochemical indicators were detected.Results The weekly water intake in the 2%met group and ovx+2%met group was significantly lower than that in the con group(P<0.01),while there was no statistically significant difference in the weekly water intake between the 2%met group and ovx+2%met group(P>0.05).At the 10th week of feeding,the serum level of Hcy in the 2%met group and ovx+2%met group was significantly higher than that in the con group(P<0.01);at the 12th week of feeding,serum Hcy>10μmol/L in the rats fed by 2%Met,and the H-type hypertensive rat model was successfully constructed.In 2 weeks after feeding,the serum level of E2 in the ovx+2%met group was significantly lower than that in the con group and 2%met group(P<0.01).SBP at each time point in the 2%met group and ovx+2%met group was higher than that in the con group,moreover,the differences from 8 weeks after feeding were statistically significant(P<0.05 or 0.01),but there was no statistical difference in SBP at each time point between the ovx+2%met group and 2%met group(P>0.05).Conclusion OVX in SHR combined with drinking 2%Met water for 12 weeks could build a menopausal H-type hypertensive rat model.

关 键 词：H型高血压 卵巢 蛋氨酸 更年期 大鼠 动物模型 

分 类 号：R544.1[医药卫生—心血管疾病]