CD4细胞内ATP浓度代替CD4/CD8比值用于评估结核感染患者的免疫状态  

Intracellular ATP Concentrations of CD4 Cells Is Better than the CD4/CD8 Ratio for Assessing the Immune Statuses of TB Patients

作　　者：刘振杰 林东子[3] 涂玉佩 周丹 李玉美 楼建荣 杨翔 徐宁 LIU Zhen-jie;LIN Dong-zi;TU Yu-pei;ZHOU Dan;LI Yu-mei;LOU Jian-rong;YANG Xiang;XU Ning(The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510370,China;Guangdong Provincial Hospital of Chinese Medicine,Guangzhou 510370,China;Foshan Fourth People’s Hospital,Foshan 528200,China;Leide Biosciences Co.,Ltd,Guangzhou 510670,China;Dongguan Sixth People's Hospital,Dongguan 523000,China)

机构地区：[1]广州中医药大学第二附属医院检验医学部,广东广州510370 [2]广东省中医院检验医学部,广东广州510370 [3]佛山市南海区第四人民医院,广东佛山528200 [4]广州市雷德生物科技有限公司,广东广州510670 [5]东莞市第六人民医院,广东东莞523000

出　　处：《中山大学学报（医学科学版）》2022年第5期748-756,共9页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广州市民政局科技计划项目(2021MZK24);广东省中医药管理局中医药科研项目(20222058);广东省珠江人才计划(00201512);广州开发区创业和创新领军人才(二期)(2021-L033);羊城创新创业领军人才支持计划(2016011)。

摘　　要：【目的】:研究CD4 T淋巴细胞内的基础ATP浓度代替CD4/CD 8比值用于评估结核病感染者免疫状态的可行性。【方法】:本研究通过磁珠法分离相同体积全血中的CD4、CD 8细胞,并计算CD4和CD 8细胞的基础ATP浓度来评估结核病感染者的免疫状态,以QFM(Quantiferon Monitor)检测法作为对照。【结果】:研究发现传统的CD4/CD 8比值或CD4 T细胞计数方法不适用于结核病患者。采用CD4细胞的基础ATP浓度来监测活动性结核患者和健康人群,在以290.7×10^(-6)g/L为cut-off值,AUROC为0.8719,其灵敏度为86.21%,特异性为76.19%,差异有统计学意义(P<0.0001)。【结论】:本研究首次使用非外界刺激条件下CD4+T淋巴细胞胞内基础ATP浓度来评估结核病感染者的免疫状态。提示CD4+T淋巴细胞的基础ATP浓度比CD4/CD 8比值可以更好用于评估结核病患者的免疫状态。【Objective】To investigate the feasibility of basal ATP concentration in CD4 T lymphocytes instead of CD4/CD 8 ratio in assessing the immune status of tuberculosis-infected patients.【Methods】In this study,the CD4 and CD 8 cells in the same volume of whole blood were separated by magnetic beads method,and the basal ATP concentration of CD4 and CD 8 cells was calculated to evaluate the immune status of tuberculosis-infected patients.Quantiferon Monitor(QFM)assay was used as a control.【Results】Our study confirmed that traditional CD4/CD 8 ratio andCD4 T cell counting methodswerenot suitable for TB patients.Using the basal ATP concentration of CD4 cells to monitor active tuberculosis pa⁃tients and healthy people,when the cut-off value was 290.7×10^(-6) g/L,the sensitivity was 86.21%,and the specificity was 76.19%,and the difference was statistically significant(P<0.0001).【Conclussions】In this study,to the best of our knowledge,we have for the first time used the basal ATP concentration in CD4+T lymphocytes to evaluate the immune sta⁃tus of tuberculosis-infected patients under non-external stimulation conditions.It is suggested that the basal ATP concen⁃tration of CD4+T lymphocytes can be better used to evaluate the immune status of tuberculosis patients than CD4/CD 8 ratio.

关 键 词：结核分枝杆菌 基础ATP CD4 T淋巴细胞 免疫状态 

分 类 号：R446[医药卫生—诊断学]