水稻芽期响应镉胁迫的转录组分析  

Transcriptome Analysis of Rice Bud Stage in Response to Cadmium Stress

作　　者：王燕宁 黄涛[1] 吴光亮 黄诗颖 钟奇 王鹏[1] 杨朦朦 李才敬 程琴 贺浩华[1,2] 金伟 郭凌 边建民 WANG Yanning;HUANG Tao;WU Guangliang;HUANG Shiying;ZHONG Qi;WANG Peng;YANG Mengmeng;LI Caijing;CHENG Qin;HE Haohua;JIN Wei;GUO Ling;BIAN Jianmin(Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Nanchang 330045,China;Jiangxi Super Rice Engineering Technology Research Center,Nanchang 330045,China;Nanchang Agricultural Technology Promotion Center,Nanchang 330200,China)

机构地区：[1]作物生理生态与遗传育种教育部重点实验室,江西南昌330045 [2]江西省水稻高水平工程研究中心,江西南昌330045 [3]南昌市农业技术推广中心,江西南昌330200

出　　处：《华北农学报》2022年第5期25-35,共11页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(32160485);国家级大学生创新创业训练计划项目(202210410002)。

摘　　要：为研究不同类型水稻对镉(Cd)胁迫差异的原因,以镉敏感籼稻恢复系昌恢891(CH891)和镉耐受粳稻品种02428为材料,选取种子萌发后,连续镉处理3 d的和未进行镉处理的幼芽,使用高通量Illumina HiSeq 4000测序技术进行RNA-Seq分析,共得到539524490个有效读长,与参考基因组的比对率在94.81%~96.82%,GC含量均在49%以上。通过比较分析,共检测到7204个差异表达基因(DEGs),其中在CH891中特异表达的基因(SCR3)有849个,02428中特异表达的基因(RCR3)有676个,2个品种均有表达但表达量存在差异的基因(CCR3)有770个。KEGG和GO富集通路分析表明,异黄酮生物合成、磷酸肌醇代谢、黄酮类生物合成和花青素生物合成途径在SCR3中显著富集,细胞核、自噬调节和胰岛素抗性途径在RCR3中显著富集,α-亚麻酸代谢、吞噬体、柠檬烯和蒎烯降解和脂肪酸降解途径在CCR3中显著富集。结果表明,Cd处理后,次级代谢过程主要在CH891中富集,蛋白质代谢主要在02428中富集,综上所述,CH891和02428响应的代谢路径存在差异,控制这些代谢路径的分子机制可能是造成不同水稻镉胁迫差异的主要原因。In order to study the reasons for the differences of different types of rice under cadmium stress,it used the cadmium sensitive indica rice restorer line Changhui 891(CH891)and the cadmium tolerant japonica rice variety 02428 as materials,and selected the buds after seed germination,which were continuously treated with cadmium for 3 days and not treated with cadmium for RNA-Seq analysis using high-throughput Illumina HiSeq 4000 sequencing technology.A total of 539524490 valid read were obtained,the comparison rate with the reference genome was between 94.81%and 96.82%,and the GC content was above 49%.Through comparative analysis,a total of 7204 differentially expressed genes(DEGs)were detected,among which 849 genes were specifically expressed in CH891(SCR3),676 genes were specifically expressed in 02428(RCR3),and 770 genes were expressed in both varieties(CCR3),but the expression level was different.The analysis of enrichment pathways of KEGG and GO showed that isoflavone biosynthesis,inositol phosphate metabolism,flavonoid biosynthesis and anthocyanin biosynthesis were significantly enriched in SCR3.Ribosome,regulation of autophagy and insulin resistance pathways were significantly enriched in RCR3.Alpha-linolenic acid metabolism,phagosome,limonene and pinene degradation and fatty acid degradation pathways were significantly enriched in CCR3.The results showed that after Cd treatment,the secondary metabolic process was mainly enriched in CH891,and the protein metabolism was mainly enriched in 02428.In conclusion,the metabolic pathways of CH891 and 02428 were different after Cd treatment,and the molecular mechanism controlling these metabolic pathways might be the main reason for the differences of cadmium stress in different rice varieties.

关 键 词：籼稻 粳稻 镉胁迫 转录组 差异表达 

分 类 号：S511.03[农业科学—作物学] Q78[生物学—分子生物学]