Colonization of Penicillium oxalicum SL2 in Pb-contaminated paddy soil and its immobilization effect on soil Pb  

作　　者：Yu Gao Xiaohan Jiang Hanxin Wu Jianhao Tong Xinyue Ren Jiayu Ren Qianhua Wu Jien Ye Chunhui Li Jiyan Shi 

机构地区：[1]Department of Environmental Engineering,College of Environmental and Resource Science,Zhejiang University,Hangzhou 310058,China [2]MOE Key Laboratory of Environmental Remediation and Ecological Health,College of Environmental and Resource Science,Zhejiang University,Hangzhou 310058,China

出　　处：《Journal of Environmental Sciences》2022年第10期53-62,共10页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 42077119);the Innovative Research Group Project of the National Natural Science Foundation of China (No. 41721001)。

摘　　要：Penicillium oxalicum SL2(SL2) is a previously screened Pb-tolerant fungus that can promote crops growth. The relationship between SL2 colonization and Pb immobilization was studied to provide a theoretical basis for microbial remediation of Pb-contaminated paddy soil.In this study, green fluorescent protein(GFP) labeled SL2 was inoculated into different Pbcontaminated paddy soils(S1-S6). The Pb extracted from the soil by HNO, EDTA and CaClwere used to characterize the available Pb. The results showed that the colonization of SL2was divided into lag phase(0-7 days), growth phase(7-30 days), and mortality phase(30-90days). SL2 colonized well in sandy soils rich in clay and total phosphorus with initial p H of 4.5-7.0. In addition, SL2 increased soil p H and decreased soil Eh, which was beneficial to immobilize Pb. In different soils, the highest percentages of CaCl-Pb, EDTA-Pb, and HNO3-Pb immobilized by SL2 were 34.34%-40.53%, 17.05%-20.11%, and 7.39%-15.62%, respectively.Pearson correlation analysis showed that the percentages of CaCl-Pb and EDTA-Pb immobilized by SL2 were significantly positively correlated with the number of SL2 during the growth phase. SL2 mainly immobilized Pb in the growth phase and a higher peak number of SL2 was beneficial to the immobilization of Pb.

关 键 词：Penicillium oxalicum SL2 COLONIZATION Lead Availability Green fluorescent protein 

分 类 号：X53[环境科学与工程—环境工程] X172