荧光探针在半胱氨酸检测的应用  

Fluorescent Probes for Cysteine Detection

作　　者：周宇航 丁莎 夏勇 刘跃军[1] Yuhang Zhou;Sha Ding;Yong Xia;Yuejun Liu(School of Packaging and Materials Engineering,Hunan University of Technology,Zhuzhou 412007,China)

机构地区：[1]湖南工业大学包装与材料工程学院,株洲412007

出　　处：《化学进展》2022年第8期1831-1862,共32页Progress in Chemistry

基　　金：湖南省教育厅科研项目(No.20C0593)资助。

摘　　要：半胱氨酸(Cys)是三种生物硫醇之一,是20种天然氨基酸中唯一一种含还原性巯基的天然氨基酸,是组成细胞内多肽和蛋白质的基本氨基酸之一。其参与体内细胞的氧化还原调控,调节体内氧化还原平衡,维持机体正常代谢,在生理过程中发挥着至关重要的作用。然而体内的Cys浓度水平异常会引起一系列生理疾病,体内的Cys浓度作为几种疾病的生物标志物具有临床意义。因此有效地识别和检测半胱氨酸受到越来越多的研究者们的青睐。相较传统检测方法,荧光探针因其操作简单、灵敏度高、响应迅速和实时检测等优点,已被广泛用于检测生物硫醇。本文基于常见荧光团的结构性能特征,综述了近三年来检测Cys的荧光探针,重点概述了其传感机制,并对其生物应用进行了简要说明,展望了未来Cys探针的研究方向与应用前景。Cysteine(Cys)is one of the three biological thiols.It is the only natural amino acid containing a reducing sulfhydryl group among the twenty natural amino acids.It is one of the basic amino acids that composes intracellular polypeptides and proteins.It participates in redox regulation of cells,regulates the redox balance in the body,and maintains the normal metabolism of the body,which plays a vital role in the physiological process.However,abnormal levels of Cys concentration in the body can cause a series of physiological diseases,and the concentration of Cys in the body has clinical significance as a biomarker of several diseases.Therefore,the effective identification and detection of cysteine is favored by more and more researchers.Compared with traditional detection methods,fluorescent probes have been widely used to detect biological thiols because of their simple operation,high sensitivity,rapid response and real-time detection.Based on the structural and performance characteristics of common fluorophores,this article reviews the fluorescent probes used to detect Cys in the past three years,focuses on their sensing mechanisms,briefly describes its biological applications,and prospects the future research directions and application prospects of Cys probes.

关 键 词：半胱氨酸 荧光探针 荧光团 检测 

分 类 号：O657.3[理学—分析化学]