鄱阳湖沙地不同植被恢复措施下土壤细菌群落特征  

Characteristics of bacterial community in Poyang Lake sandy land under different vegetation restoration conditions

作　　者：杨贤房[1,2] 郑林 马永杰[1] YANG Xianfang;ZHENG Lin;MA Yongjie(School of Geography and Environment,Jiangxi Normal University,Nanchang 330022,China;School of Geography and Environment,Gannan Normal University,Ganzhou 341000,China)

机构地区：[1]江西师范大学地理与环境学院,南昌330022 [2]赣南师范大学地理与环境工程学院,赣州341000

出　　处：《应用与环境生物学报》2022年第4期1027-1033,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(41661114);江西省教育厅科技落地计划项目和江西省教育厅科学技术研究项目(190763)资助。

摘　　要：为掌握不同植被恢复措施下鄱阳湖沙地土壤细菌群落变化特征,以湿地松、香根草、单叶蔓荆、狗牙根等植物恢复措施的沙地土壤为研究对象,采用高通量测序技术,分析植物恢复前后细菌群落变化.结果显示,相较对照组,土壤细菌多样性均有提升,在门水平上,绿弯菌门丰度大幅降低,而变形菌门、放线菌门和厚壁菌门丰度增加.在属水平上,副伯克霍尔德氏菌属、慢生根瘤菌属、芽孢杆菌属、分枝杆菌属等丰度提升,而假单胞菌属丰度大幅下降.植被恢复后,不同植被固氮功能细菌丰度提升,而香根草、狗牙根土壤磷细菌丰度下降,湿地松土壤固氮、解磷功能优势细菌提升幅度为76.11%、51.12%,单叶蔓荆为65.04%、23.17%.土壤容重、有机质、全磷、有效氮是影响功能细菌变化的重要因素,其中有效氮与芽孢杆菌属、分枝杆菌属呈显著水平以上相关性.本研究表明鄱阳湖沙地植被恢复措施提升了固氮细菌的丰富度,但不同植被土壤解磷细菌群落变化差异大;研究结果可为沙地治理提供科学依据.(图6表3参34)To understand the changes in the bacterial community in the Poyang Lake sandy land under different vegetation restoration conditions, the soil under the canopies of Pinus elliottii, vetiver grass, Vitex trifolia, and bermudagrass were taken for analysis. High-throughput sequencing technology was used to explore changes in the bacterial community after restoration. At the phylum level, the abundance of Chloroflexi decreased significantly, while the abundance of Actinobacteria, Proteobacteria, and Firmicutes increased. At the genus level, the abundance of dominant functional bacteria, such as Paraburkholderia, Bradyrhizobium, Bacillus, and Mycobacteria, increased significantly whereas the abundance of Pseudomonas decreased. In general, with vegetation restoration, the abundance of nitrogen-fixing bacteria increased, whereas the abundance of phosphatesolubilizing bacteria decreased in vetiver grass and bermudagrass soils. The dominant nitrogen-fixing bacteria and phosphate-solubilizing bacteria increased by 76.11% and 51.12% in Pinus elliottii soil, and 65.04% and 23.17% in Vitex trifolia soil, respectively. The results of the correlation analysis showed that soil bulk density, organic matter,total phosphorus, and available nitrogen were important factors affecting the change in functional bacteria. The results showed that vegetation restoration measures improved the abundance of nitrogen-fixing bacteria in the Poyang Lake sandy land, but the phosphate-solubilizing bacterial communities varied in different vegetation soils.The conclusions of this study can provide a scientific basis for sandy-land management.

关 键 词：高通量测序 鄱阳湖沙地 细菌群落 植被恢复 固氮细菌 解磷细菌 

分 类 号：S154.3[农业科学—土壤学]