抑郁大鼠脑区多模态磁共振影像改变及颐脑解郁方的调控作用  

Changes in multimodal MRI of related brain regions in depressed rats and regulatory effects of Yinao Jieyu Formula

作　　者：赵瑞珍 郑笑[2] 许芳 李江林 李小黎 ZHAO Ruizhen;ZHENG Xiao;XU Fang;LI Jianglin;LI Xiaoli(Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China;Beijing Hospital,Beijing 100730,China;Bejing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]北京医院 [3]北京中医药大学

出　　处：《北京中医药大学学报》2022年第9期897-902,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81673930);中央高校基本科研业务费专项重点攻关项目(No.2020-JYB-ZDGG-144-3)。

摘　　要：目的采用核磁技术观察抑郁大鼠大脑相关部位脑白质纤维束完整性、局部灌注情况等变化,从神经影像角度验证颐脑解郁方对抑郁症的正向调节作用。方法根据行为学评估结果将雄性Wistar大鼠随机分为正常组、模型组、颐脑解郁方组(6.2 g/kg)、盐酸氟西汀组(2.33 mg/kg)4组,之后采用慢性不可预知温和性刺激法构建抑郁模型,造模成功后灌胃相应药物或等量蒸馏水,每日1次,共4周。灌胃结束应用扩散张量成像(DTI)、动脉自旋标记成像(ASL)方法,测定各组大鼠各向异性比值(FA)、表观扩散系数(ADC)以及血流量(CBF)值。结果DTI显示:模型组大鼠双侧海马、前额叶皮层FA值低于正常组(P<0.05,P<0.01),ADC值高于正常组(P<0.05,P<0.01)。与模型组比较,颐脑解郁方组和盐酸氟西汀组在右侧海马及双前额叶皮层FA值升高(P<0.05,P<0.01);右侧海马及左侧前额叶皮层ADC值降低(P<0.05,P<0.01)。ASL显示:模型组左侧海马、双侧前额叶皮层CBF值较正常组降低(P<0.05,P<0.01),颐脑解郁方组和盐酸氟西汀组双侧前额叶皮层CBF值较模型组升高(P<0.05,P<0.01)。结论抑郁大鼠可见海马、前额叶皮层出现纤维束不同程度的损伤和微循环变化。颐脑解郁方可以通过改善神经微循环和物质代谢,修复受损的神经纤维束、恢复神经元完整、改善神经元活性及重塑能力等途径缓解或阻断抑郁症状。应用磁共振影像组学技术可较好地重现抑郁发展过程中的结构和功能变化,且具有一定的疗效评价意义。Objective To observe the changes in the integrity of white matter fiber bundle and local cerebral perfusion in relevant brain areas of depressed rats with magnetic resonance technique,and to verify the positive regulatory effect of Yinao Jieyu(Brain-benefiting Depression-resolving,YNJY)Formula on depression from the perspective of neuroimaging in a non-isolated experiment with depressed rats.Methods Based on the result of behavioral assessment,male Wistar rats were randomly divided into normal group,model group,YNJY group and fluoxetine hydrochloride group(FH group).And the depression model was established by chronic unpredictable mild stimulation(CUMS).Then the YNJY group was gavaged with YNJY decoction,the FH group with fluoxetine hydrochloride capsule,and the other two groups with distilled water for four weeks.After that,magnetic resonance diffusion tensor imaging(DTI)and arterial spin labeling(ASL)were performed to measure the fractional anisotropy(FA),apparent diffusion coefficient(ADC)and cerebral blood flow(CBF)values of each group,so as to obtain the nerve fiber structure and local cerebral perfusion level of the corresponding areas in rats.Results DTI showed that in bilateral hippocampus and prefrontal cortex in the model group,the FA values were lower(P<0.05,P<0.01),and the ADC values were significantly higher compared with those of the normal group(P<0.05,P<0.01).Compared with the model group,FA values in the YNJY and FH groups were significantly higher in the right hippocampus and bilateral prefrontal cortex(P<0.05,P<0.01),and ADC values were lower in the left prefrontal cortex(P<0.05,P<0.01).(2)The result of ASL imaging showed that the CBF values in the left hippocampus and bilateral prefrontal cortex regions of the brain were lower in the model group compared with those of the normal group(P<0.05,P<0.01),and the CBF values in the bilateral prefrontal cortex were significantly higher in the YNJY and FH groups compared with those of the model group(P<0.05).Conclusion Fiber bundle damage of varying de

关 键 词：磁共振成像 抑郁 颐脑解郁方 扩散张量成像 动脉自旋标记 大鼠 

分 类 号：R285.5[医药卫生—中药学]