芍药GGPPS家族基因的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of GGPPS Gene Family from Paeonia lactiflora Pall.

作　　者：左永丽 吴疆[2] 温雅欣 徐杰 史国安[1] 范丙友[1] Zuo Yongli;Wu Jiang;Wen Yaxin;Xu Jie;Shi Guoan;Fan Bingyou(College of Agriculture,Henan University of Science and Technology,Luoyang,471003;School of Information Engineering,Yulin University,Yulin,719000;CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences Shanghai,200032)

机构地区：[1]河南科技大学农学院,洛阳471003 [2]榆林学院信息工程学院,榆林719000 [3]中国科学院分子植物科学卓越创新中心,上海200032

出　　处：《分子植物育种》2022年第18期5962-5973,共12页Molecular Plant Breeding

基　　金：国家自然科学基金项目(U1204323);河南省高等学校重点科研项目(20A210010)共同资助。

摘　　要：萜类物质是芍药根的重要药用活性成分,香叶基香叶基焦磷酸合酶(geranylgeranyl pyrophosphate synthase,GGPPS)是萜类物质生物合成的关键酶。为研究GGPPS基因在芍药根萜类物质生物合成中的作用,本研究基于芍药转录组测序数据(DRX027794),从芍药根中成功克隆了PlGGPPS家族的3个基因并对其进行了生物信息学分析。结果表明,PlGGPPS、PlGGPPSssu-like和PlGGPPSssu基因(GenBank登录号分别为KP708574,KP708573和KP708572)分别编码含有369、298和340个氨基酸的蛋白质,它们与多种近缘植物GGPPS蛋白家族成员具高同源性。预测的蛋白质均隶属于萜类合酶超级家族,然而PlGGPPSssu-like蛋白缺少保守的FARM和SARM保守结构域,分子进化树分析结构显示其分类地位相对独立。蛋白亚细胞定位于叶绿体或质体,为不含跨膜结构域、不含信号肽的不稳定蛋白;其二级结构主要以α-螺旋和无规则卷曲为主,三级结构以单体形式存在,为后续开展PlGGPPS家族中PlGGPPS、PlGGPPSssu-like和PlGGPPSssu基因的时空表达特性分析及其功能研究提供了理论基础。Terpenoids are an important medicinal active ingredient in the root of Paeonia lactiflora and geranylgeranyl pyrophosphate synthase is one of the key enzymes in terpenoids biosynthesis.In order to study the function of GGPPS gene in the biosynthesis of terpenoids in P.lactiflora,three members of PlGGPPS gene family were successfully cloned from root of P.lactiflora based on the transcriptome sequencing data(DRX027794)and analyzed by bioinformatic methods.It showed that PlGGPPS,PlGGPPSssu-like and PlGGPPSssu(KP708574,KP708573 and KP708572)encoded 369,298 and 340 amino acids,which had high homology with members of GGPPS protein family in a variety of related plants.The predicted proteins belonged to the terpene synthase superfamily,but PlGGPPSssu-like protein lacked the conserved FARM and SARM domains.The phylogenetic tree analysis indicated that the status of Pl GGPPSssu-like protein was relatively distant.Protein were predicted to be located in the chloroplast or plastid,and all of them were unstable proteins without transmembrane domains or signal peptides.Their secondary structures were mainly consisted ofα-helix and random coils and 3D structures existed as monomer.It provided a theoretical foundation for the following study of the temporal-spatial expression analysis and the function characterization of PlGGPPS,PlGGPPSssu-like and PlGGPPSssu gene in PlGGPPS family.

关 键 词：芍药(Paeonia lactiflora) 香叶基香叶基焦磷酸合酶 萜类物质 基因克隆 生物信息学分析 

分 类 号：S682.12[农业科学—观赏园艺]