雪茄烟遗传图谱构建及叶面重要性状的QTL定位  

Construction of Genetic Map and QTL Mapping of Important Leaf Traits in Cigar

作　　者：蒋勋 宋健 刘国祥 王俊 范静苑 李媛 王琰琰 戴培刚[1] 张兴伟[1] Jiang Xun;Song Jian;Liu Guoxiang;Wang Jun;Fan Jingyuan;Li Yuan;Wang Yanyan;DaiPeigang;Zhang Xingwei(Institute of Tobacco Research,Chinese Academy of Agricultural Sciences,Qingdao,266101;Taicang Agricultural and Rural Bureau,Taicang Agricultural and Forestry Administration Comprehensive Law Enforcement Team,Taicang,215400;Sichuan Tobacco Company Deyang Company,Deyang,618400;Great Wall Cigar Factory of Sichuan China Tobacco Industry Co.,LTD.,Shifang,618400)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]太仓市农业农村局太仓市农林行政综合执法大队,太仓215400 [3]四川省烟草公司德阳市公司,德阳618400 [4]四川中烟工业有限责任公司长城雪茄烟厂,什邡618400

出　　处：《分子植物育种》2022年第18期6076-6086,共11页Molecular Plant Breeding

基　　金：中国烟草总公司海南省公司科技项目(201846000024055);中国烟草总公司四川省公司科技项目(SCYC-201903)共同资助。

摘　　要：目前,中国雪茄烟市场消费持续增长,国产优质雪茄茄衣原料供应严重不足,针对茄衣的遗传基础研究相对较少。叶面褶皱度、叶片厚薄、支脉粗细和主支脉夹角是雪茄烟茄衣重要性状,对茄衣品质有重要影响。本研究通过构建‘Beinhart1000-1’ב鹤峰把把烟’F_(2)和F_(2∶3)遗传群体,基于简化基因组测序构建出一张覆盖24条连锁群,包含1036个SNP标记的雪茄烟遗传连锁图谱,对三个发育期的叶面褶皱度、叶片厚薄、支脉粗细和主支脉夹角4个性状进行QTL定位,检测到66个相关的QTL。其中4个叶面褶皱度QTL在多个环境中被重复检测到,位于1号、14号和20号连锁群;2个叶面褶皱度QTL在多个环境中被重复检测到,位于2号和22号连锁群;3个支脉粗细QTL在多个环境中被重复检测到,位于7号、8号连锁群;1个主支脉夹角QTL在多个环境中被重复检测到,位于18号连锁群上。这些重要位点的发掘为下一步继续开展茄衣烟叶叶面重要性状相关基因的图位克隆和分子标记的开发提供了一定的理论基础。At present,the domestic cigar market continues to grow,the domestic high quality cigar eggplant garment raw material supply is seriously insufficient,regarding the eggplant garment genetic basic research is relatively few.The degree of leaf fold,leaf thickness,branch thickness and main branch angle are the important characters of cigar tobacco,which have important influence on the quality of cigar tobacco.In this study,by constructing’BeinHart1000-1’×’Hefengbabayan’Fand Fgenetic populations,a cigar genetic linkage map covering 24 linkage groups and containing 1036 SNP markers was constructed based on simplified genome sequencing.66 related QTLs were detected in three development stages of four traits:leaf fold degree,leaf thickness,branch thickness and main branch angle.Among them,four QTLs related to foliar fold degree were repeatedly detected in multiple environments and were located in linkage groups 1,groups 14 and groups 20.Two QTLs related to leaf fold degree were repeatedly detected in multiple environments and were located in linkage groups 2 and groups 22.QTLs related to the thickness of three branches were repeatedly detected in multiple environments,located in linkage groups 7,groups 8.QTLs associated with the angle of one main branch were detected repeatedly in multiple environments and were located in linkage group No.18.The discovery of these important loci laid a good theoretical foundation for the further development of marker development and mapping cloning of genes related to important traits in tobacco leaves and molecular marker-assisted selection breeding.

关 键 词：雪茄烟 QTL 简化基因组测序 遗传图谱 

分 类 号：TS453[农业科学—烟草工业]