杨树叶枯病原菌多基因鉴定及对不同杨树品种致病性  

Polygenic Identification of Poplar Leaf Blight Pathogen and Its Pathogenicity to Different Poplar Varieties

作　　者：冯连荣[1,2] 赵大根 矫丽曼 宋立志 彭儒胜 Feng Lianrong;Zhao Dagen;Jiao Liman;Song Lizhi;Peng Rusheng(School of Forestry,Northeast Forestry University,Harbin,150040;Liaoning Provincial Institute of Poplar,Gaizhou,115213)

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]辽宁省杨树研究所,盖州115213

出　　处：《分子植物育种》2022年第18期6097-6103,共7页Molecular Plant Breeding

基　　金：辽宁省自然科学基金项目(2021-MS-052)资助。

摘　　要：对发生在中荷64号杨(Populus×euramericana cv‘N3016’)上的叶枯病进行了病原物的分离培养,经形态学观察结合分子生物学技术进行了ITS(核糖体DNA内部转录间区)、HIS(组蛋白)、ACT(肌动蛋白)、EF1-α(延伸因子)多基因联合鉴定及柯赫氏法则验证,确定引起中荷64号杨叶枯病的病原菌为链格孢菌(Alternaria alternata)。利用离体叶片接种方法,开展了链格孢菌对山杨(P.davidiana)、欧洲黑杨(P.×nigra‘N1’)、小叶杨(P.simonii Carr.)、辽胡2号杨(P.simonii×P.euphratica‘Liaohu2’)的致病性研究,结果显示链格孢菌可以引起4种杨树不同程度发病,其中山杨、欧洲黑杨发病严重,辽胡2号杨、小叶杨发病较轻。本研究为杨树叶枯病的田间诊断、杨树抗病品种筛选提供了理论依据。The pathogen of leaf blight was isolated and cultured in the Populus×euramericana cv.’N3016’,Joint identification of multiple genes and verification of Koch’s rule in ITS(Internal transcribed region of ribosomal DNA),HIS(Histone),ACT(Agonist protein)and EF1-α(Elongation factor)by morphological observation combined with molecular biology technology,the leaf blight pathogen of Populus×euramericana cv.’N3016’was Alternaria alternata.Using the inoculation method of isolated leaves,the pathogenicity of Alternaria alternata to P.davidiana,P.×nigra’N1’,P.simonii Carr.and P.simonii×P.euphratica’Liaohu2’were studied.The results showed that Alternaria alternata could cause different degrees of disease in four poplar species,P.davidiana and P.×nigra’N1’were seriously illness,P.simonii×P.euphratica’Liaohu2’and P.simonii Carr.were light illness.This study provides a theoretical basis for the field diagnosis of poplar leaf blight and the selection of poplar resistant varieties.

关 键 词：杨树 叶枯病 链格孢菌 致病性 

分 类 号：S763.7[农业科学—森林保护学]