活血通降方对反流性食管炎大鼠肠道菌群及Caspase-3/GSDME通路的影响  

Effects of Huoxue Tongjiang Prescription on intestinal flora and cysteine protease 3/gasdermin E pathway in rats with reflux esophagitis

作　　者：尹红 唐艳萍[2] 杨磊[2] 刘思雨 YIN Hong;TANG Yanping;YANG Lei;LIU Siyu(Graduate School of Tianjin University of Traditional Chinese Medicine,Tianin,301617,China;Department of GastroenterologyⅡ,Tianjin Nankai Hospital)

机构地区：[1]天津中医药大学研究生院,天津301617 [2]天津市南开医院消化内科二

出　　处：《中国中西医结合消化杂志》2022年第10期701-707,712,共8页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：国家自然科学基金资助项目(No:81573737,No:82074213)。

摘　　要：目的:探讨活血通降方对反流性食管炎大鼠肠道菌群及半胱氨酸蛋白酶3/焦孔素E(Caspase-3/GSDME)通路的影响。方法:将30只Wistar大鼠随机分为对照组、模型组、活血通降方组,每组10只。除对照组外,其余两组均采用改良部分贲门肌切开术+外置幽门部分结扎术建立反流性食管炎大鼠模型。造模成功后,对照组及模型组予2.5 mL生理盐水灌胃,活血通降方组予2.5 mL活血通降方(3.68 g/kg)灌胃,2次/d,连续给药14 d。采用苏木精-伊红染色法观察各组大鼠食管组织病理变化;采用蛋白组学检测差异蛋白变化;采用酶联免疫吸附法检测血清中内毒素、IL-1β和IL-18的表达;采用蛋白免疫印迹法检测食管组织中Caspase-3和GSDME的表达,采用16S rDNA高通量测序检测肠道菌群的变化。结果:模型组大鼠食管组织病理评分较对照组增高(P<0.05),活血通降方组大鼠食管组织病理评分较模型组下降(P<0.05);蛋白组学分析表明,Caspase-3是各组的主要差异蛋白;与对照组比较,模型组肠道菌群的物种丰度显著降低,未分类拟杆菌属、Prevotellaceae_UCG-003属、螺旋菌属及Prevotellaceae_UCG-001属等革兰阴性菌的相对丰度显著增加,Akkermansia与Ruminococcaceae_UCG-005属的相对丰度显著降低,血清中内毒素、IL-1β和IL-18的含量及食管组织中Caspase-3和GSDME蛋白的表达明显升高(P<0.05,P<0.01);与模型组比较,活血通降方可改善肠道菌群组成,显著增加有益菌Ruminococcaceae_UCG-005的丰度,降低革兰阴性菌如Prevotellaceae_UCG-003属、拟杆菌属及未分类拟杆菌属的相对丰度,显著降低血清中内毒素、IL-1β和IL-18的含量,且可明显下调食管组织中Caspase-3和GSDME蛋白的表达水平(P<0.05,P<0.01)。结论:活血通降方可能通过调节肠道菌群降低体内内毒素水平,从而下调食管组织中Caspase-3和GSDME蛋白的表达及降低焦亡相关炎症因子的释放,对反流性食管炎大鼠黏膜损伤起�Objective:To investigate the effects of Huoxue Tongjiang Prescription on intestinal flora and cysteine protease 3/gasdermin E(Caspase-3/GSDME)pathway in rats with reflux esophagitis.Methods:Thirty Wistar rats were randomly divided into control group(control group),model group(RE group)and Huoxue Tongjiang Prescription group,with 10 rats in each group.Except for the control group,all other groups used modified partial cardia myotomy and external pyloric partial ligation to establish RE rat models.After successful modeling,the control group and the Model group were given 2.5 mL normal saline intragastric administration,Huoxue Tongjiang Prescription group was given 2.5 mL Huoxue Tongjiang Prescription(3.68 g/kg)intragastric administration,twice a day for 14 days.The pathological changes of esophagus tissues were observed by hematoxylin-eosin(HE)staining.The changes of differential proteins were detected by proteomics.The expression of LPS,IL-1β,and IL-18 in serum was detected by Enzyme-linked immunosorbent assay(ELISA).The expression of Caspase-3 and GSDME in esophageal tissues was detected by protein immunoblotting.The changes of intestinal flora were detected by 16 S rDNA high-throughput sequencing.Results:Compared with the control group,the esophageal histopathological score of rats in the model group increased(P<0.05);compared with the model group,the esophageal histopathological score of rats in the Huoxue Tongjiang Prescription group decreased(P<0.05);proteomics analysis showed that Caspase-3 was the main differential protein in each group;compared with the control group,the species richness of the intestinal flora in the model group was significantly reduced,and the relative abundance of Gram-negative bacteria such as unclassified Bacteroides,Prevotellaceae_UCG-003,Helicobacter,and Prevotellaceae_UCG-001 were significantly increased.The relative abundance of Akkermansia and Ruminococcaceae_UCG-005 decreased significantly,the contents of LPS,IL-1β,and IL-18 in serum,and the protein expression of Caspase-3 an

关 键 词：反流性食管炎 活血通降方 细胞焦亡 肠道菌群 脂多糖 半胱氨酸蛋白酶3 焦孔素E 信号通路 

分 类 号：R473.5[医药卫生—护理学]