HSRP1携氧液对兔控制性重度失血性贫血早期灌注的疗效观察  

Effect of HSRP1 oxygen-carrying solution on early perfusion in rabbits with controlled severe hemorrhagic anemia

作　　者：游可为 刘嘉馨[1] 李剑波 王文刚 YOU Kewei;LIU Jiaxin;LI Jianbo;WANG Wengang(Institute of Blood Transfusion,Chinese Academy of Medical Sciences and Peking Union Medical College,Chengdu 610052,China;Redpharm(Beijing)Biopharmaceutical Institute Co.,Ltd.)

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052 [2]润方(北京)生物医药研究院有限公司

出　　处：《中国输血杂志》2022年第9期911-914,共4页Chinese Journal of Blood Transfusion

摘　　要：目的观察戊二醛聚合牛血红蛋白注射液(glutaraldehyde polymerizes bovine hemoglobin injection,代号:HSRP1)在兔重度失血性贫血早期灌注的疗效。方法建立兔控制性重度失血性贫血模型,将制模成功的12只兔分为HSRP1组和乳酸钠林格注射液(sodium lactate ringer's injection,LR)组,每组6只,雌雄各半。HSPR1组和LR组分别用HSRP1和LR进行治疗。观察两组实验兔的存活率,并测量失血前后、灌注后24 h内各个时间点的血流动力学、静脉血气、血浆血红蛋白,以及碱剩余、乳酸和碳酸氢根等指标。结果HSRP1组实验兔存活率与LR组差异具有统计学意义(P<0.05);放血后,各组实验兔的平均动脉压显著降低,差异具有统计学意义(P<0.05),但在灌注后HSPR1组实验兔的平均动脉压与LR组差异无统计学意义;HSRP1组实验兔在灌注第二阶段,血液乳酸浓度和碱剩余在各时间点与LR组差异均存在统计学意义(P<0.05),在灌注后2 h时,呼吸频率与LR组差异均存在统计学意义(P<0.05),在灌注后4 h时心率与LR组相比,差异存在统计学意义(P<0.05);HSRP1组实验兔的血浆静脉氧分压、静脉氧饱和度和血浆血红蛋白在实验的各个时间点与LR组差异,均无统计学意义。结论HSPR1可用于兔重度失血性贫血,通过对缺氧组织供氧,纠正无氧代谢,提高实验兔的存活率。HSPR1作为一种新型携氧液,早期可纠正重度失血性贫血患者的氧供平衡。Objective To observe the effect of glutaraldehyde polymerized bovine hemoglobin injection(code:HSRP1)oxygen-carrying fluid on early perfusion of severe hemorrhagic anemia in rabbits.Methods The rabbit model of controlled severe hemorrhagic anemia was established.Twelve modeled rabbits were divided into glutaraldehyde polymerized bovine hemoglobin injection(code:HSRP1)group and sodium lactate ringer's injection(LR)group,with 6 rabbits in each group(half male and half female).HSPR1 group and LR group were treated with HSRP1 and LR,respectively.The survival rate of experimental rabbits was observed,and the indexes of hemodynamics,venous blood gas,plasma hemoglobin,base surplus,lactic acid and bicarbonate were measured before and after blood loss,as well as each point within 24 h after infusion.Results The survival rate of HSRP1 group was significantly different from that of LR group(P<0.05);After exsanguination,the mean arterial pressure of each group was significantly different from that before exsanguination(P<0.05),but there was no significant difference between HSPR1 group and LR group after infusion;In the second stage of perfusion,the blood lactate concentration and base excess in the HSRP1 group were significantly different from those in the LR group at each time point(P<0.05),at 2 h after perfusion,the respiratory rate started to differ significantly from that of the LR group(P<0.05),heart rate was significantly different from LR group at 4 h after perfusion(P<0.05);There were no significant differences between HSRP1 group and LR group in plasma venous oxygen partial pressure,venous oxygen saturation and plasma hemoglobin at all time points.Conclusion HSPR1 is used for severe traumatic hemorrhagic shock in rabbits,and can improve the survival rate of experimental rabbits by providing oxygen to hypoxic tissues and correcting anaerobic metabolism.As a new oxygen-carrying fluid,HSPR1 can correct the oxygen supply balance of patients with severe hemorrhagicanemia in early stage.

关 键 词：贫血 失血性贫血 戊二醛聚合牛血红蛋白 血红蛋白氧载体 乳酸钠林格液 

分 类 号：R556.1[医药卫生—血液循环系统疾病]