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作 者:荣小涵 文浩杰 周娟 崔毅 唐金勇 Rong Xiaohan;Wen Haojie;Zhou Juan;Cui Yi;Tang Jinyong(Department of Otolaryngology and Head Surgery,the First People’s Hospital of Chenzhou,the Affiliated Chenzhou Hospital of Southern Medical University,Hunan Chenzhou 423000,China)
机构地区:[1]郴州市第一人民医院,南方医科大学附属郴州医院耳鼻咽喉头颈外科,湖南郴州423000
出 处:《遵义医科大学学报》2022年第5期591-597,共7页Journal of Zunyi Medical University
基 金:郴州市科技局科研发展计划项目(NO:zdyf201829)。
摘 要:目的探究GLRX3在鼻咽癌(NPC)中的表达及其对铁代谢的影响和相关通路的调控。方法利用免疫组化检测鼻咽癌组织与癌旁组织中GLRX3的表达水平;采用RT-PCR检测组织中GLRX3 mRNA的表达水平;将人鼻咽癌上皮细胞HONE1设置为4组,分别为未转染的细胞对照组(Control组),转染对照序列shRNA-NC的细胞阴性对照组(shNC组),转染序列shRNA-GLRX3的细胞组(shGLRX3组),铁离子螯合剂处理细胞组(DFO组);RT-PCR与Western blot检测各组细胞中GLRX3、Ferritin、TFR1、Wnt1、β-catenin的mRNA和蛋白表达水平;采用CCK-8法和Transwell法分别检测上述各组细胞的增殖和侵袭能力。结果与癌旁组织相比,鼻咽癌组织中GLRX3 mRNA的表达显著增加(P<0.05)。与Control组和shNC组相比较,shGLRX3组细胞中GLRX3 mRNA及蛋白的表达水平均显著下调(P<0.05),前两组无明显差异(P>0.05)。同时,shGLRX3组铁代谢相关蛋白Ferritin、TFR1的表达较Control组显著降低(P<0.01);shGLRX3组与DFO组细胞的增殖与侵袭能力较Control组细胞均显著降低(P<0.01),前两组无明显差异(P>0.05)。且shGLRX3组细胞和DFO组细胞中Wnt1、β-catenin的mRNA及蛋白的表达也显著降低(P<0.05)。结论降低鼻咽癌中高表达的GLRX3可能通过下调肿瘤细胞的铁代谢相关蛋白,抑制细胞的增殖与侵袭能力,这可能与Wnt/β-catenin信号通路的激活相关。Objective To investigate the expression of GLRX3 in nasopharyngeal carcinoma(NPC)and its influence on iron metabolism and related pathways.Methods Immunohistochemistry staining was used to detect the expression level of GLRX3 in NPC tissues and adjacent tissues.RT-PCR was performed to measurethe expression of GLRX3 mRNA in the tissues.The human NPC epithelial cell HONE1 was divided into 4 groups:control group,negative control group of cells transfected with control sequence shRNA-NC(shNC group),group of cells transfected with sequence shRNA-GLRX3(shGLRX3group)and cell group treated with iron ion chelator(DFO group).RT-PCR and Western blot assay were applied to detect the mRNA and protein expressionsof GLRX3,Ferritin,TFR1,Wnt1 andβ-catenin.CCK-8 method and transwell method were used to detect cell proliferation and invasion.Results Compared with adjacent tissues,the expression of GLRX3 mRNA in NPC tissues was increased.Compared with the control group and the shNC group,the expression of GLRX3 mRNA and protein in the shGLRX3 group was down-regulated.At the same time,the expressions of iron metabolism-related proteins Ferritin and TFR1 in shGLRX3 group were lower than those in control group.The proliferation and invasion ability of cells in shGLRX3 and DFO groups were lower than those in control group.The expressions of Wnt1 andβ-catenin mRNA and protein in shGLRX3 group and DFO group cells were also reduced.Conclusion Decreasing the high expression of GLRX3 in NPC could inhibit the proliferation and invasion of tumor cells by down-regulating the iron metabolism-related proteins of tumor cells,which mightbe related to the activation of Wnt/β-catenin signaling pathway.
关 键 词:鼻咽癌 GLRX3 铁代谢 铁离子螯合剂 WNT/Β-CATENIN信号通路
分 类 号:R541.4[医药卫生—心血管疾病]
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