贵州4株花脸香蘑的分离鉴定及秋栽驯化  

Isolation,Identification and Autumn Cultivation of Four Strains of Lepista sordida in Guizhou

作　　者：张秀伟 鲍菊 张万萍[2] 蔡甫格 牛力立 ZHANG Xiu-wei;BAO Ju;ZHANG Wan-ping;CAI Fu-ge;NIU Li-li(Anshun Academy of Agricultural Sciences,Anshun 561000,China;College of Agriculture,Guizhou University,Guiyang 550025,China)

机构地区：[1]安顺市农业科学院,贵州安顺561000 [2]贵州大学农学院,贵州贵阳550025

出　　处：《中国食用菌》2022年第10期26-30,共5页Edible Fungi of China

基　　金：2019年贵州省基础研究项目(黔科合基础[2019]1001号);2019年贵州省科技支撑计划重点项目(黔科合支撑[2019]2451-4号)。

摘　　要：以采自贵州黔中地区的4株野生大型真菌(NK、LM、JUF、GS)为研究材料,形态学结合ITS测序进行品种鉴定,并利用茶树枝叶发酵料对其进行秋栽驯化试验。鉴定结果表明,菌株NK、LM、JUF、GS均为花脸香蘑,同源性达99%。以茶树枝叶发酵料栽培4个花脸香蘑菌株发现,其菌丝萌发速度快,发菌时间短。出菇试验中,菌株GS、NK、LM的生物学效率分别达24.5%、25.6%、10.3%;当温度降至10℃时,3个菌株的菇蕾不再生长,因此在黔中地区适宜9月中旬播种,可以采收二潮菇。Taking four strains of wild macrofungi(NK,LM,JUF,GS) collected from Guizhou Province as research materials,the varieties were identified by morphology combined with ITS sequencing,and the fermentation material made of tea tree sawdust tea were used for autumn cultivation and domestication test.The identification results showed that NK,LM,JUF and GS were all Lepista sordida with 99% homology.It was found that the mycelial germination rate was faster and the germinating time was short when the four L.sordida strains cultivated with tea sawdust fermentation material.In the fruiting test,the biological efficiencies of GS,NK and LM were 24.5%,25.6% and 10.3% respectively.When the temperature drops to about 10 ℃,the mushroom buds no longer grow,so it is suitable to sown in the middle of september in Guizhou,two flush mushrooms can be harvested.

关 键 词：贵州花脸香蘑 分离鉴定 秋栽驯化 

分 类 号：S646.1[农业科学—蔬菜学]