葛根素减轻LPS诱导的小鼠肾脏炎性反应  

Puerarin attenuates LPS-induced renal inflammatory response of mice

作　　者：冯娜 唐杰 贺细菊 田宗滢 史丹丹 余晶晶 FENG Na;TANG Jie;HE Xi-ju;TIAN Zong-ying;SHI Dan-dan;YU Jing-jing(Department of Anatomy,Basic Medical College,Hubei University of Medicine,Shiyan 442000;Renmin Hospital,Hubei University of Medicine,Shiyan 442000,CHina)

机构地区：[1]湖北医药学院基础医学院人体解剖学教研室,湖北十堰442000 [2]湖北医药学院附属人民医院,湖北十堰442000

出　　处：《基础医学与临床》2022年第11期1727-1730,共4页Basic and Clinical Medicine

基　　金：湖北省卫生计生委科研项目(WJ2017 M213)。

摘　　要：目的探讨葛根素(PUE)是否减轻脂多糖(LPS)诱导的小鼠炎性反应。方法选择30名健康体检者为对照(control)组,45例严重感染(SI)非肾损伤患者作为N-SI组,30例严重感染致肾损伤患者作SI组,收集患者外周血样本。将50只小鼠随机分为对照(Ctrl)组,LPS组,LPS+PUE(10、25、50 mg/kg·d,ip)组,PUE在给予LPS后1 h应用,24 h后处死小鼠,采集血液。ELISA检测血清Scr、BUN、IL-6和IL-33水平。结果与对照组和N-SI组相比,SI组患者血清中BUN、Scr、IL-6和IL-33水平显著升高(P<0.05)。小鼠LPS组与对照组相比,血清中肾功能指标BUN和Scr均显著增高(P<0.05);与LPS组相比,PUE可显著降低BUN、Scr、MDA、IL-33和IL-6的表达水平,并使SOD的活性升高(P<0.05)。结论PUE可减轻LPS诱导的小鼠炎性反应。Objective To investigate whether puerarin(PUE)can alleviate lipopolysarccharide(LPS)-induced renal inflammatory response in mice.Methods Thirty health control,45 severe infection with no renal injury(N-SI group),30 severe infection with renal injury(SI group)were enrolled in this study,Serum samples were collected.50 mice were randomly divided into 5 groups,including control(Ctrl)group,LPS group,and LPS+PUE(10,25,50 mg/kg·d,IP)groups.PUE was applied 1 h after LPS administration.And mice were sacrificed after 24 h and blood sample was collected.MDA,SOD,IL-33 and IL-6 expression were detected by ELISA.Results BUN,Scr,IL-6 and IL-33 in SI group were significantly increased compared with control group and N-SI group(P<0.05).BUN and Scr of LPS group were significantly increased compared with control group.PUE decreased BUN,Scr,MDA,IL-33 and IL-6 expression and increased activity of SOD as compared with LPS group(P<0.05).Conclusions PUE can alleviate LPS-induced renal inflammatory response in mice.

关 键 词：葛根素 脂多糖 炎性反应 

分 类 号：R692[医药卫生—泌尿科学]