白心与黄心糖木薯代谢物及相关基因表达分析  

Analysis of Metabolites and Related Gene Expression of Sugar Cassava with White and Yellow Fleshes

作　　者：安飞飞[1] 罗秀芹[1] 薛晶晶[1] 陈松笔[1] 蔡杰 AN Feifei;LUO Xiuqin;XUE Jingjing;CHEN Songbi;CAI Jie(Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava/Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业部木薯种质资源保护与利用重点实验室,海南海口571101

出　　处：《江西农业大学学报》2022年第5期1092-1100,共9页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家重点研发计划项目(2018YFD1000501);海南省自然科学基金项目(320MS100)。

摘　　要：【目的】木薯是世界十亿人的口粮,选育高营养型品种是木薯育种的重要目标之一。通过对白心和黄心糖木薯薯肉代谢物及基因表达分析明确其差异,为高营养型木薯品种改良提供理论依据。【方法】采用超高液相色谱(UPLC)分析薯肉β-胡萝卜素含量,气相色谱质谱联用技术(GC-MS)分析薯肉糖含量,液相色谱质谱联用技术(LC-MS)分析薯肉代谢物,并对差异代谢物进行通路富集分析,采用qRT-PCR对关键基因的表达进行分析。【结果】黄心糖木薯β-胡萝卜素含量、淀粉含量、半乳糖含量、果糖含量显著高于白心木薯,而蔗糖含量和海藻糖含量显著低于白心木薯,葡萄糖含量与白心糖木薯无显著差异。以白心糖木薯为对照,利用代谢组学方法检测到2 719种代谢产物,其中267种代谢物发生显著变化,其中黄心糖木薯中上调差异代谢物163个,下调104个,主要涉及能量代谢、脂肪酸代谢、氨基酸代谢、苯丙烷生物合成、淀粉和糖代谢以及类黄酮代谢。267种差异代谢物被注释到74个代谢通路中,其中59种差异代谢物显著或极显著影响18个代谢通路。前10位发生极显著改变的代谢通路分别是亚油酸代谢、α-亚麻酸代谢、ABC转运、丙氨酸、天冬氨酸和谷氨酸代谢、丙酮酸代谢、氨酰tRNA生物合成、氧化磷酸化、碳代谢、乙醛酸和二羧酸代谢和TCA循环。与白心糖木薯相比,黄心糖木薯中淀粉合成相关基因表达上调,淀粉降解相关基因表达下调,β-胡萝卜素合成相关基因均上调。【结论】黄心糖木薯薯肉β-胡萝卜素、淀粉、半乳糖和果糖含量高,而白心糖木薯薯肉中蔗糖和海藻糖含量高。黄心糖木薯脂肪酸代谢途径、淀粉及半乳糖代谢途径、苯丙烷生物合成及类黄酮代谢更活跃,而白心糖木薯氨基酸代谢途径和能量代谢途径更活跃。[Objective]Since cassava is the staple food for nearly one billion people in the world,the breeding of high-nutrition cassava varieties becomes the top priority of cassava breeding.This study aims to analyze the metabolites of flesh in both white sugar cassava and yellow sugar cassava,and clarify the differential metabolites and gene expression,which provideds a theoretical basis for genetic improvement of high-nutrient cassava.[Method]The content of β-carotene in cassava flesh was determined by ultra high liquid chromatography(UPLC),the sugar content was measured by gas chromatography-mass spectrometry(GC-MS),and the metabolites were in the fleshes of white and yellow cassava were detected by liquid chromatography-mass spectrometry(LC-MS),in which the enriched pathways were annotated,real-time fluorescence quantitative PCR(qRT-PCR)was used in this study to analyze the expression of the genes.[Result]The contents of β-carotene,starch,galactose and fructose in yellow flesh cassava were significantly higher than those in white flesh cassava,however,the sucrose and trehalose contents in yellow flesh cassava were significantly lower than those in white flesh cassava,and there was no significant difference in glucose content between both cassava fleshes.2 719 metabolites were detected and 267 differential metabolites in yellow flesh cassava were identified compared with white cassava.Among them,163 metabolites were up regulated and 104 were down regulated.The differential metabolites mainly involved in energy,fatty acid,amino acid,phenylpropane biosynthesis,starch,sugar and flavonoid metabolism.267 metabolites were annotated into 74 metabolic pathways,59 metabolites significantly or extremely significantly affected 18 metabolic pathways.The top 10 metabolic pathways included linoleic acid metabolism,α-linolenic acid metabolism,ABC transport,alanine,aspartic acid and glutamate metabolism,pyruvic acid metabolism,aminoacyltrna biosynthesis,phosphorylation,carbon metabolism,glyoxylic acid and dicarboxylic acid metaboli

关 键 词：木薯 薯肉 差异代谢物 代谢通路 基因 

分 类 号：S533[农业科学—作物学]