环核苷酸门控离子通道基因CNGC3正调控拟南芥抗核盘菌免疫  

Cyclic nucleotide-gated ion channel gene CNGC3 positively regulates immunity against Sclerotinia sclerotiorum in Arabidopsis

作　　者：刘梦娇 易航 蔡新忠[1,2] LIU Mengjiao;YI Hang;CAI Xinzhong(Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang University/Zhejiang Provincial Key Laboratory of Crop Pathogen and Insect Biology,Hangzhou 310058,China;Hainan Institute,Zhejiang University,Sanya 572025,Hainan,China)

机构地区：[1]浙江大学农业与生物技术学院生物技术研究所/浙江省作物病虫生物学重点实验室,杭州310058 [2]浙江大学海南研究院,海南三亚572025

出　　处：《浙江大学学报（农业与生命科学版）》2022年第5期594-604,共11页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省自然科学基金项目(LZ18C140002);浙江省农业新品种选育重大科技专项(2021C02064);国家自然科学基金项目(31871947)。

摘　　要：环核苷酸门控离子通道(cyclic nucleotide-gated ion channel,CNGC)对死体营养型病原物的免疫调控功能及机制尚知之甚少。在本研究中,通过基因产物亚细胞定位、基因表达模式以及免疫调控功能的遗传学分析,探究了AtCNGC3基因对典型死体营养型病原物核盘菌(Sclerotinia sclerotiorum)的免疫调控功能。结果表明:AtCNGC3蛋白定位于质膜上。AtCNGC3基因在拟南芥各组织中均有表达,没有组织特异性。核盘菌接种强烈且持续诱导AtCNGC3基因的表达。Atcngc3突变体植株对核盘菌更易感,而AtCNGC3基因超表达(AtCNGC3 overexpression,AtCNGC3-OE)植株表现出更强的核盘菌抗性,说明AtCNGC3正调控拟南芥对核盘菌的抗性。用植物激发子肽AtPep1浸泡处理拟南芥幼苗能迅速诱导AtCNGC3基因表达,AtPep1诱导的活性氧(reactive oxygen species,ROS)迸发在Atcngc3突变体植株中减弱,而在AtCNGC3-OE植株中增强,说明AtCNGC3正调控AtPep1诱导的ROS迸发。综上所述,本研究结果揭示了AtCNGC3正调控拟南芥对死体营养型病原真菌(核盘菌)的免疫,增进了对CNGC免疫调控功能的认识。Functions and mechanisms of cyclic nucleotide-gated ion channel(CNGC)in plant immunity against necrotrophic pathogens remain largely unknown.In this study,we explored the functions of AtCNGC3 in immunity against Sclerotinia sclerotiorum,a typical necrotrophic pathogen,through identifying subcellular localization of its expression product,defining its expression patterns and genetically analyzing its function in immunity.The results showed that AtCNGC3 protein was localized in plasma membrane.AtCNGC3 was highly expressed in all analyzed tissues of Arabidopsis thaliana without showing any tissue specificity.Sclerotinia sclerotiorum inoculation strongly and constantly induced the expression of AtCNGC3 gene.The Atcngc3 mutant plants were more susceptible to S.sclerotiorum,while AtCNGC3 overexpression(AtCNGC3-OE)plants exhibited enhanced resistance to S.sclerotiorum,indicating that AtCNGC3 positively regulates the resistance to S.sclerotiorum.Seedlings soaking with Arabidopsis thaliana plant elicitor peptide 1(AtPep1)could rapidly induce AtCNGC3 gene expression.AtPep1-elicited reactive oxygen species(ROS)burst was weakened in the Atcngc3 mutant seedlings while enhanced in the AtCNGC3-OE plants,demonstrating that AtCNGC3 positively regulates AtPep1-induced ROS burst.Collectively,our results reveal that AtCNGC3 positively regulates immunity against the necrotrophic fungal pathogen S.sclerotiorum in Arabidopsis,thereby providing some insights into the functions of CNGC in plant immunity.

关 键 词：环核苷酸门控离子通道 核盘菌 抗病性 植物免疫 活性氧 

分 类 号：S432[农业科学—植物病理学]