紫草阳和汤对肉芽肿小叶性乳腺炎大鼠caspase-1、GSDMD、IL-1β及雌孕激素受体的影响  

Effect of Zicao Yanghe Decoction on Caspase-1/GSDMD/IL-1βand Estrogen and Progesterone Receptors in Granulomatous Lobular Mastitis

作　　者：刘洁丽 史晓光[2] 汪唐顺[2] 左禧萌 冯雪[2] 王玉坤 高爽 孙萍 LIU Jie-li;SHI Xiao-guang;WANG Tang-shun;ZUO Xi-meng;FENG Xue;WANG Yu-kun;GAO Shuang;SUN Ping(Beijing University of Chinese Medicine,Beijing 100700;Department of General Surgery,Dongzhimen Hospital,Bei-jing University of Chinese Medicine,Beijing 100029)

机构地区：[1]北京中医药大学,北京100700 [2]北京中医药大学东直门医院普外科,北京100029

出　　处：《世界中西医结合杂志》2022年第8期1551-1557,共7页World Journal of Integrated Traditional and Western Medicine

基　　金：北京中医药大学2021年度新教师启动基金项目(2021-BUCMXJKT001)。

摘　　要：目的探讨紫草阳和汤对肉芽肿小叶性乳腺炎大鼠的疗效及炎症指标、细胞焦亡相关蛋白及雌孕激素受体的作用。方法将20只SPF级雌性SD大鼠随机分为空白对照组、模型组、西药组、紫草阳和汤组,每组各5只,对模型组、西药组及紫草阳和汤组大鼠进行肉芽肿小叶性乳腺炎造模,并于次日开始,予西药组1次/d,10 mg/kg醋酸泼尼松,予紫草阳和汤组1次/d,1.85 g/kg紫草阳和汤,21 d后处死取材。乳腺组织进行病理染色、免疫组化染色法测定雌激素受体(Estrogen receptors,ER)、孕激素受体(Progesterone receptors,PR)表达强度、Elisa法测定各组大鼠血清IL-18表达、Western Blot法测定乳腺组织细胞焦亡有关蛋白[全长消皮素D(GasderminD-fulllenth,GSDMD-fl)、消皮素D-N端蛋白(GasderminD-N,GSDMD-N)、全长天冬氨酸蛋白水解酶1(Cysteinyl aspartate specific proteinase-1 fulllenth,caspase1-fl)、活化的天冬氨酸蛋白水解酶1(Cleavedcysteinyl aspartate specific proteinase 1,cleaved caspase-1)、活化的白细胞介素1β(Cleaved-interleukin 1β,cleaved IL-1β)]表达,测量大鼠第3、4对乳腺直径及大鼠体重。结果(1)与模型组比较,紫草阳和汤组与西药组均出现了炎性细胞浸润,紫草阳和汤组PR表达明显下降(P<0.05),而ER表达差异无统计学意义;(2)与模型组比较,紫草阳和汤组IL-18明显下降(P<0.05);(3)与模型组比较:紫草阳和汤组cleaved caspase-1及cleaved IL-1β表达水平降低(P<0.05),GSDMD-N明显降低(P<0.001);西药组cleaved caspase-1表达水平明显降低(P<0.05),GSDMD-N明显降低(P<0.001);(4)与模型组比较,紫草阳和汤组与西药组出现了乳房直径下降。结论紫草阳和汤对肉芽肿小叶性乳腺炎大鼠具有一定疗效,主要表现为对IL-18、IL-1β、cleaved caspase-1及GSDMD-N的影响,并可下调PR的表达。Objective To investigate the effect of ZicaoYanghe Decoction on curative efficacy,inflammatory indexes,pyroptosis-related proteins,estrogen receptor(ER)and progesterone receptor(PR)of granulomatous lobular mastitis rats.Methods Twenty rats were randomly divided into blank control group,model group,western medicine group and ZicaoYanghe Decoction group,with 5 in each group.The model of granulomatous lobular mastitis was established in rats of three groups except the blank control group.From the next day of modeling,the rats in the western medicine group were given 10mg/kg prednisone once daily,and those in ZicaoYanghe Decoction group were treated with 1.85 g/kg ZicaoYanghe Decoction once a day.The rats were fed for 21 days and then sacrificed.The breast tissue was stained for pathological observation,and the expression of ER and PR in breast tissue was detected by immunohistochemistry.The expression of interleukin-18(IL-18)in serum and pyroptosis-related proteins[gasdermin D-full lenth(GSDMD-fl),gasdermin D-N(GSDMD-N),cysteinyl aspartate specific proteinase-1 full lenth(caspase1-fl),cleaved cysteinyl aspartate specific proteinase-1(cleaved caspase-1),cleaved-interleukin 1β(cleaved IL-1]in breast tissue was detected by enzyme-linked immunosorbent assay(ELISA)and Western blot,respectively.The diameter of the 3^(rd) and 4^(th) pair mammary glands and the weight of the rats were measured.Results Compared with the conditions in the model group,(1)there was inflammatory cell infiltration in both ZicaoYanghe Decoction group and western medicine group,and the expression of PR in ZicaoYanghe Decoction group was decreased(P<0.05),while the expression of ER had no significant difference;(2)the content of IL-18 in ZicaoYanghe Decoction group dropped(P<0.05);(3)the expression levels of cleaved Caspase-1 and cleaved IL-1β(P<0.05)and GSDMD-N(P<0.001)in the ZicaoYanghe Decoction group were lowered,and those of cleaved Caspase-1(P<0.05)and GSDMD-N(P<0.001)in the western medicine group were also reduced;(4)the breast diameter was

关 键 词：肉芽肿小叶性乳腺炎 紫草阳和汤 白细胞介素-1Β 雌激素受体 孕激素受体 

分 类 号：R285.5[医药卫生—中药学]